Dyl'ovyĭ M V
Mikrobiol Z. 1995 Jan-Feb;57(1):44-7.
Cells of Escherichia coli K 12 grown on nutritious broth and twice washed off from medium by 90 mM solution of lithium chloride alkalize to some degree acidic non-nutritional medium. Registration of rates of alkalization by the cells at constant pH (from 3.0 to 5.5) was made by means of automatic potentiometric titration. The initial rate of alkalization was higher at low pH of the incubation medium. Different mechanisms of medium alkalization by bacterial cells (consumption of protons by cells, antiport proton/metal ion and release of basic products by cells) are discussed.
在营养肉汤中生长的大肠杆菌K12细胞，用90 mM氯化锂溶液从培养基中洗涤两次后，会使酸性非营养培养基在一定程度上碱化。通过自动电位滴定法记录细胞在恒定pH值（3.0至5.5）下的碱化速率。在孵育培养基的低pH值下，碱化的初始速率较高。文中讨论了细菌细胞使培养基碱化的不同机制（细胞消耗质子、质子/金属离子反向转运以及细胞释放碱性产物）。
