Mooradian A D, Uko-eninn A
St. Louis VA Medical Center, MO, USA.
Brain Res. 1995 Feb 6;671(1):159-63. doi: 10.1016/0006-8993(94)01326-d.
To determine if aging in rats is associated with increased susceptibility of cerebral microvessels to oxidative damage microvessels from the cerebrum of 4-, 12-, 18- and 26-month-old male Fischer 344 rats were studied. The malondialdehyde (MDA) (micrograms/mg protein) content of cerebral microvessels from 12-month-old rats (0.032 +/- 0.002) was significantly higher than that in 4-month-old rats (0.020 +/- 0.015) P < 0.01. The difference between 26-month-old (0.025 +/- 0.002) and 4-month-old rats did not reach statistical significance. The antioxidative potential was measured in the presence of a peroxy radical generator 2,2'-azobis(2-amidionopropane)hydrochloride (AAPH) with monitoring of the fluorescence of phycoerythrin at 37 degrees C. The free radical quenching activity of cerebral microvessels expressed as % inhibition of phycoerythrin oxidation by AAPH was significantly reduced in 12-month-old (33.6 +/- 4.6%) and 18-month-old rats (26.9 +/- 1.4%) compared with 4-month-old rats (54.3 +/- 4.9%) (P < 0.01). The 26-month-old rats (46.4 +/- 4.6%) were not significantly different from 4-month-old rats. It is concluded that aging is associated with increased lipid peroxidation byproducts in cerebral microvessels along with a transient decrease in their antioxidative capacity.
为了确定大鼠衰老是否与脑微血管对氧化损伤的易感性增加有关，对4、12、18和26月龄雄性Fischer 344大鼠大脑中的微血管进行了研究。12月龄大鼠脑微血管中丙二醛（MDA）（微克/毫克蛋白）含量（0.032±0.002）显著高于4月龄大鼠（0.020±0.015），P<0.01。26月龄大鼠（0.025±0.002）与4月龄大鼠之间的差异未达到统计学显著性。在过氧自由基发生器2,2'-偶氮双（2-脒基丙烷）盐酸盐（AAPH）存在的情况下，于37℃监测藻红蛋白荧光，测定抗氧化潜力。以AAPH对藻红蛋白氧化的抑制百分比表示的脑微血管自由基淬灭活性在12月龄（33.6±4.6%）和18月龄大鼠（26.9±1.4%）中显著低于4月龄大鼠（54.3±4.9%）（P<0.01）。26月龄大鼠（46.4±4.6%）与4月龄大鼠无显著差异。结论是，衰老与脑微血管中脂质过氧化产物增加以及其抗氧化能力短暂下降有关。
