Mooradian A D, Meredith K E
St. Louis VA Medical Center, Department of Medicine, MO.
Neurochem Res. 1992 Jul;17(7):665-70. doi: 10.1007/BF00968002.
The effect of age on protein composition of cerebral microvessels was investigated by examining the content of glycosylation endproducts in cerebral microvessels isolated from young (3-6 month old), intermediate age (18 month) and aged (24-26 month old) Fischer 344 male rats and by quantitating various protein spots identified with two dimensional (2D) electrophoresis. The results indicate that aging in rats is not associated with significant increase in glycosylation of microvessel proteins. Of the 26 proteins in cerebral microvessels identified on the 2-D gel, ten showed significant age-related changes (p less than 0.0004) and in two of these the changes were significant as early as 18-months of age. A large acidic protein with a molecular weight of 144,000 and isoelectric point (pI) of 5.4 (Spot #1) was found only in aged rats. The results indicate that aging is associated with significant quantitative changes in protein composition of cerebral microvessels. It is possible that Spot #1 may be a novel biochemical marker of aging blood-brain barrier.
通过检测从年轻（3 - 6个月大）、中年（18个月）和老年（24 - 26个月大）的Fischer 344雄性大鼠分离出的脑微血管中糖基化终产物的含量，并对二维（2D）电泳鉴定出的各种蛋白质斑点进行定量分析，研究年龄对脑微血管蛋白质组成的影响。结果表明，大鼠衰老与微血管蛋白质糖基化显著增加无关。在二维凝胶上鉴定出的脑微血管中的26种蛋白质中，有10种显示出与年龄相关的显著变化（p小于0.0004），其中两种早在18个月龄时变化就很显著。一种分子量为144,000、等电点（pI）为5.4的大酸性蛋白质（斑点#1）仅在老年大鼠中发现。结果表明，衰老与脑微血管蛋白质组成的显著定量变化有关。斑点#1可能是衰老血脑屏障的一种新型生化标志物。
