Riederer K M, Lawson P, Held M S, Petrylka K, Briski L E, Khatib R
St. John Hospital and Medical Center, Detroit, MI 48236-2172.
Can J Microbiol. 1995 Jan;41(1):88-91. doi: 10.1139/m95-011.
Latex agglutination and the enzyme immunoassays Cytoclone (EIA-C) and VIDAS (EIA-V) were compared with a cytotoxicity assay for the diagnosis of Clostridium difficile associated diarrhea. Among patients with discrepant results, the cytotoxicity assay and clinical assessment were used to evaluate the performance of the latex agglutination and EIA tests. Clostridium difficile associated diarrhea was documented in 30/149 samples (20.1%) from 130 patients. All test results matched in 113 instances. Latex agglutination, EIA-C, and EIA-V yielded false positive results in 10, 4, and 7 samples and false negative results in 8, 9, and 14 samples, respectively. Latex agglutination demonstrated 87.8% efficiency compared with 91.3% for EIA-C and 85.7% for EIA-V and 3 min hands-on time compared with 4.5 min for EIA-V and 10 min for EIA-C. On the basis of these findings and given the fact that all rapid tests have their shortcomings, we believe that latex agglutination is the most practical method.
将乳胶凝集试验以及酶免疫测定法（细胞克隆法[EIA - C]和VIDAS法[EIA - V]）与细胞毒性试验进行比较，以诊断艰难梭菌相关性腹泻。在结果不一致的患者中，采用细胞毒性试验和临床评估来评价乳胶凝集试验和酶免疫测定法的性能。130例患者的149份样本中有30份（20.1%）记录为艰难梭菌相关性腹泻。113例中所有检测结果均相符。乳胶凝集试验、EIA - C和EIA - V分别在10份、4份和7份样本中出现假阳性结果，在8份、9份和14份样本中出现假阴性结果。乳胶凝集试验的效率为87.8%，EIA - C为91.3%，EIA - V为85.7%；乳胶凝集试验的实际操作时间为3分钟，EIA - V为4.5分钟，EIA - C为10分钟。基于这些发现，并且考虑到所有快速检测方法都有其缺点，我们认为乳胶凝集试验是最实用的方法。
