Sekulić M, Lovren M, Popović T
Institute for Biological Research, Division of Cytology and Embryology, Belgrade, Yugoslavia.
Experientia. 1995 Apr 15;51(4):335-8. doi: 10.1007/BF01928890.
Beta-thyrotropin (TSH)-producing cells in the pituitary pars distalis of female rats were studied using rabbit anti-rat beta-thyrotropin (TSH) serum and a peroxidase-antiperoxidase (PAP) immunohistochemical procedure. Animals were neonatally treated with 1 mg estradiol-dipropionate (EDP) and sacrificed at different stages of development up to adulthood. Intact females of the corresponding age served as the controls. Morphometry and stereology were used to evaluate the changes in TSH-cell number and volume densities of the cells and nuclei. All morphometric parameters examined in estradiol-treated animals showed a significant decrease in comparison with immunoreactive TSH cells of age-matched controls. The most prominent EDP-induced changes were evident in peripubertal 38-day-old rats, the number and volumetric densities of both TSH cells and their nuclei being reduced by about 90% compared to intact pituitary. This decrease in the number and volume densities of TSH cells in EDP-treated rats explicitly demonstrated that this hormone, applied neonatally, has an inhibitory effect on TSH-immunoreactive cells up to adulthood, in accordance with our earlier data obtained by light and electron microscopy.
使用兔抗大鼠β-促甲状腺激素（TSH）血清和过氧化物酶-抗过氧化物酶（PAP）免疫组织化学方法，对雌性大鼠垂体远侧部中产生β-促甲状腺激素（TSH）的细胞进行了研究。动物在新生期用1毫克二丙酸雌二醇（EDP）处理，并在发育至成年期的不同阶段处死。相应年龄的完整雌性动物作为对照。采用形态计量学和体视学方法评估TSH细胞数量以及细胞和细胞核的体积密度变化。与年龄匹配的对照动物的免疫反应性TSH细胞相比，在经雌二醇处理的动物中检测的所有形态计量学参数均显示出显著降低。最明显的EDP诱导变化在青春期前38日龄大鼠中可见，与完整垂体相比，TSH细胞及其细胞核的数量和体积密度均降低了约90%。EDP处理大鼠中TSH细胞数量和体积密度的这种降低明确表明，新生期应用这种激素对TSH免疫反应性细胞具有抑制作用，直至成年期，这与我们早期通过光学显微镜和电子显微镜获得的数据一致。
