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A new member of the cytosolic O-acetylserine(thiol)lyase gene family in Arabidopsis thaliana.

作者信息

Barroso C, Vega J M, Gotor C

机构信息

Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC y Universidad de Sevilla, Spain.

出版信息

FEBS Lett. 1995 Apr 17;363(1-2):1-5. doi: 10.1016/0014-5793(95)00255-8.

DOI:10.1016/0014-5793(95)00255-8
PMID:7729527
Abstract

A cDNA, Atcys-3A, encoding O-acetylserine-(thiol)lyase has been isolated from Arabidopsis thaliana. The deduced peptide sequence showed a high level of similarity with the bacterial counterpart, and a remarkable percentage of identity with other higher plant O-acetylserine(thiol)lyase genes. Sequence comparison and Southern blot analysis suggested that Atcys-3A was a new and different to the previously reported member of the cytosolic gene family in Arabidopsis. The Atcys-3A expression was activated by sulfur limitation, requiring a carbon and nitrogen source for maximal expression. A similar pattern of regulation was observed at the O-acetylserine(thiol)lyase activity level. Northern blot analysis also showed an organ-specific expression of Atcys-3A.

摘要

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