Hell R, Bork C, Bogdanova N, Frolov I, Hauschild R
Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität Bochum, Germany.
FEBS Lett. 1994 Sep 5;351(2):257-62. doi: 10.1016/0014-5793(94)00872-8.
cDNAs encoding for two isoforms of O-acetylserine (thiol) lyase (OAS-TL), which catalyzes the synthesis of cysteine, have been isolated from Arabidopsis thaliana. Secondary structure together with expression patterns derived during photomorphogenesis indicate cellular localizations in the cytosol and plastids, thus allowing a direct comparison of compartment-specific forms within one species. The cytosolic OAS-TL complemented an E. coli auxotrophic mutant lacking cysteine synthesis. Both isoforms are represented by small gene families. They are expressed under all conditions investigated and were observed to increase in expression in plants grown with limited sulfate supply.
编码催化半胱氨酸合成的O-乙酰丝氨酸（硫醇）裂解酶（OAS-TL）两种同工型的cDNA已从拟南芥中分离出来。光形态建成过程中的二级结构以及表达模式表明其在细胞质和质体中的细胞定位，从而能够对同一物种内特定区室形式进行直接比较。胞质OAS-TL补充了缺乏半胱氨酸合成的大肠杆菌营养缺陷型突变体。两种同工型均由小基因家族代表。它们在所有研究条件下均有表达，并且在硫酸盐供应有限的情况下生长的植物中观察到表达增加。
