Ramesh V, Xu Y Z, Roberts G C
Department of Biochemistry and Biological NMR Centre, University of Leicester, UK.
FEBS Lett. 1995 Apr 17;363(1-2):61-4. doi: 10.1016/0014-5793(95)00226-y.
A convenient and economical method is described for the site-specific 15N-labelling of the 4-amino group of individual cytidine residues in oligonucleotides. This is applied to a 20 base-pair oligonucleotide corresponding to the trp operator; this oligonucleotide and its complex with the E. coli trp repressor are studied by NMR, using 1H-15N HMQC and 15N-edited 1H-1H NOESY experiments.
