Twal W, Roze L, Zile M H
Department of Food Science and Human Nutrition, Michigan State University, East Lansing 48824, USA.
Dev Biol. 1995 Apr;168(2):225-34. doi: 10.1006/dbio.1995.1075.
Avian cardiovascular development is vitamin A-dependent, and retinoic acid has been suggested to be active in this important developmental event. We report here that a monoclonal antibody against all-trans-retinoic acid blocks normal embryonic development in the quail causing cardiovascular abnormalities typical of avian vitamin A deficiency. In whole-mount preparations of stage 5 normal quail embryos the fluorescence associated with the antiretinoic acid monoclonal antibody localizes in Hensen's node and in caudal area. In stage 7-8 embryos fluorescence localizes in heart-forming areas as well as in head mesenchyme, in Hensen's node, in nephrotome, and in caudal area. These studies are the first to localize endogenous all-trans-retinoic acid during very early stages of normal avian development. We propose that all-trans-retinoic acid is biosynthesized in its target cells during early avian embryo-genesis and that the availability of this signal molecule is spatiotemporally regulated. We conclude that all-trans-retinoic acid or a closely related metabolite is the physiological form of vitamin A required for normal cardiovascular development and for other very early developmental events in the quail embryo.
禽类心血管发育依赖维生素A，并且有人提出视黄酸在这一重要的发育过程中具有活性。我们在此报告，一种针对全反式视黄酸的单克隆抗体可阻断鹌鹑正常的胚胎发育，导致出现禽类维生素A缺乏典型的心血管异常。在第5阶段正常鹌鹑胚胎的整装标本中，与抗视黄酸单克隆抗体相关的荧光定位于亨森结和尾侧区域。在第7 - 8阶段胚胎中，荧光定位于心脏形成区域以及头部间充质、亨森结、肾节和尾侧区域。这些研究首次在正常禽类发育的早期阶段定位了内源性全反式视黄酸。我们提出，全反式视黄酸在禽类胚胎早期发育过程中在其靶细胞中生物合成，并且这种信号分子的可用性受到时空调节。我们得出结论，全反式视黄酸或一种密切相关的代谢物是鹌鹑胚胎正常心血管发育和其他非常早期发育事件所需的维生素A的生理形式。
