Becker M H, Lassner F, Dagtekin F, Walter G F, Berger A
Klinik für Plastische, Hand- und Wiederherstellungschirurgie, Medizinischen Hochschule Hannover am Krankenhaus Oststad.
Handchir Mikrochir Plast Chir. 1995 Mar;27(2):93-7.
The aim of this study is to gain further knowledge concerning the regeneration of reinnervated, freely transplanted muscles. Therefore, we used a rat model, consisting of eight rats per group, in which the latissimus dorsi muscle was transplanted orthotopically, after a period of time of two and twelve weeks harvested, then evaluated histologically and enzyme-histochemically. As controls we used a group of non-operated muscles. At date of removal, the patency of the vascular anastomoses was checked clinically and histologically. Additionally, electrophysiological measurements and conventional and enzyme-histochemical histologies were performed. Two weeks after the free neurovascular flap transplantation, the muscle was not innervated yet, histologically a dissolved pattern of type 1 and type 2 muscle fibers was found. After twelve weeks of time, the muscles were reinnervated again, muscle contraction was positive after electrical stimulation and the typical pattern of fibers was reestablished.
本研究的目的是进一步了解再支配的游离移植肌肉的再生情况。因此，我们使用了一个大鼠模型，每组八只大鼠，将背阔肌原位移植，在两周和十二周后取出，然后进行组织学和酶组织化学评估。作为对照，我们使用了一组未手术的肌肉。在取出时，通过临床和组织学检查血管吻合的通畅情况。此外，还进行了电生理测量以及传统和酶组织化学组织学检查。游离神经血管皮瓣移植两周后，肌肉尚未重新获得神经支配，组织学上发现1型和2型肌纤维呈溶解模式。十二周后，肌肉再次获得神经支配，电刺激后肌肉收缩呈阳性，纤维的典型模式得以重建。
