奇异变形杆菌尿道上皮细胞黏附（uca）基因的分子克隆
Molecular cloning of Proteus mirabilis uroepithelial cell adherence (uca) genes.
作者信息
Cook S W, Mody N, Valle J, Hull R
机构信息
Department of Biology, Houston Baptist University, Texas 77074, USA.
出版信息
Infect Immun. 1995 May;63(5):2082-6. doi: 10.1128/iai.63.5.2082-2086.1995.
Abstract
Proteus mirabilis bacteria are a common cause of hospital-acquired urinary tract infection. In a previous study, we described a P. mirabilis fimbrial protein, UCA, that adhered to human uroepithelial cells. Genes sufficient for expression of UCA adherence were cloned into Escherichia coli K-12. E. coli bacteria that contained the uca recombinant plasmid adhered to human uroepithelial cells. In addition, the ucaA gene encoding the structural component of UCA pili was subcloned, and its DNA sequence was determined. Amino acid sequence homology (30 to 50%) was found between mature UcaA protein and pilins from pathogenic bacteria representing several genera, including E. coli F17, G, and type 1C pilins, Haemophilus M43 pilin, and a Bordetella pilin.
摘要
奇异变形杆菌是医院获得性尿路感染的常见病因。在先前的一项研究中，我们描述了一种奇异变形杆菌菌毛蛋白UCA，它可黏附于人类尿道上皮细胞。将足以表达UCA黏附功能的基因克隆到大肠杆菌K-12中。含有uca重组质粒的大肠杆菌可黏附于人类尿道上皮细胞。此外，对编码UCA菌毛结构成分的ucaA基因进行了亚克隆，并测定了其DNA序列。发现成熟的UcaA蛋白与来自几个属的病原菌菌毛蛋白之间存在氨基酸序列同源性（30%至50%），这些病原菌包括大肠杆菌F17、G菌毛和1C型菌毛、嗜血杆菌M43菌毛以及博德特氏菌菌毛。
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Contribution of Proteus mirabilis urease to persistence, urolithiasis, and acute pyelonephritis in a mouse model of ascending urinary tract infection.奇异变形杆菌脲酶在小鼠上行性尿路感染模型中对持续性感染、尿路结石形成及急性肾盂肾炎的作用
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