Seko Y, Shimokawa H, Tokoro T
Department of Ophthalmology, Tokyo Medical and Dental University School of Medicine, Japan.
Invest Ophthalmol Vis Sci. 1995 May;36(6):1183-7.
To determine factors that control ocular enlargement in experimental form-deprivation myopia and to clarify the mechanism of form-deprivation myopia.
After the left eyes of 20 chicks were monocularly occluded for 2 weeks, protein, basic fibroblast growth factor (bFGF) and transforming growth factor (TGF)-beta 2 contents in samples of constant area (circular button, diameter = 8.5 mm) in the retina-retinal pigment epithelium (RPE)-choroid and the sclera in the posterior region of control and myopic eyes were determined by enzyme-linked immunosorbent assay.
The bFGF content (per circular button) and bFGF concentration (per mg protein) were significantly lower in the sclera in the posterior region of the myopic eyes than in control eyes. The bFGF content and concentration were similar in the retina-RPE-choroid in myopic and control eyes. The TGF-beta 2 content and concentration were significantly higher in myopic eyes in both the retina-RPE-choroid and the sclera (P < 0.05).
These results are consistent with the possibility that bFGF and TGF-beta 2 regulate ocular enlargement or respond to myopiagenic mechanisms in form-deprivation myopia.
确定实验性形觉剥夺性近视中控制眼球增大的因素，并阐明形觉剥夺性近视的机制。
将20只雏鸡的左眼单眼遮盖2周后，采用酶联免疫吸附测定法测定对照眼和近视眼中视网膜-视网膜色素上皮（RPE）-脉络膜以及巩膜中恒定面积（圆形纽扣，直径 = 8.5 mm）样本中的蛋白质、碱性成纤维细胞生长因子（bFGF）和转化生长因子（TGF）-β2含量。
近视眼中后部区域巩膜中的bFGF含量（每圆形纽扣）和bFGF浓度（每毫克蛋白质）显著低于对照眼。近视眼和对照眼中视网膜-RPE-脉络膜中的bFGF含量和浓度相似。近视眼中视网膜-RPE-脉络膜和巩膜中的TGF-β2含量和浓度均显著更高（P < 0.05）。
这些结果与bFGF和TGF-β2调节眼球增大或对形觉剥夺性近视的致近视机制产生反应的可能性一致。
