Storch H, Schwenke H, Helbig W
Z Gesamte Inn Med. 1975 Dec 1;30(23):261-4.
In 27 patients with lupus erythematodes diseminatus the determinations of the LE-cells according to the macromethod (Zimmer and Hargraves) and the micromethod (Mudrik and co-workers) were compared with the demonstration of antinuclear factors according to the indirect immunofluorescence and immune enzyme technique. The sensitiveness of the two last-mentioned immunomorphological methods is somewhat larger. In these cases the size of the titre of the antinuclear factor almost always correlates positively with the number of the LE-cells. For the purpose of the initial diagnostics and the judgment of the course a morphological method cannot be renounced, since in the acute episode a high consumption of the antinuclear factor the immunological methods negatively correlate with the number of the LE-cells. The immune enzyme technique is to be recommended on account of the smaller expenditure, permanence of the preparations and high sensitiveness as alternative method of the immunofluorescence technique. In the micromethod the large variation is opposite to the advantage of the slight quantity of blood and to an always existing evaluability. Investigations of the lymphocytes of patients with lupus erythematodes disseminatus by means of the lymphocyte transformation test and the determination of the B-cells with the help of the direct immune peroxidase technique refer to the close pathogenetic connections of cellular and humoral immune reactions in this disease.
对27例播散性红斑狼疮患者，根据大分子法（齐默和哈格雷夫斯）和微量法（穆德里克及其同事）测定LE细胞，并与根据间接免疫荧光和免疫酶技术检测抗核因子进行比较。后两种免疫形态学方法的敏感性略高。在这些病例中，抗核因子滴度的大小几乎总是与LE细胞数量呈正相关。为了进行初步诊断和判断病程，不能放弃形态学方法，因为在急性发作期抗核因子大量消耗，免疫方法与LE细胞数量呈负相关。鉴于所需费用较低、标本保存性好且敏感性高，免疫酶技术作为免疫荧光技术的替代方法值得推荐。在微量法中，较大的变异性与所需血量少和始终可评估的优点相悖。通过淋巴细胞转化试验对播散性红斑狼疮患者的淋巴细胞进行研究，并借助直接免疫过氧化物酶技术测定B细胞，这表明该疾病中细胞免疫反应和体液免疫反应存在密切的发病机制联系。
