Richards R M, Xing D K, King T P
School of Pharmacy, Robert Gordon University, Aberdeen, UK.
J Appl Bacteriol. 1995 Mar;78(3):209-15. doi: 10.1111/j.1365-2672.1995.tb05018.x.
The antibacterial activity of p-aminobenzoic acid against Listeria monocytogenes, Salmonella enteritidis and Escherichia coli was compared with the activity of commonly used acidulants: formic, propionic, acetic, lactic and citric acids. Viable count evaluations and MIC determinations indicated that p-aminobenzoic acid caused greater inhibitory effects than the other organic acids. The activity of p-aminobenzoic acid on the growth of the test organisms at selected pH values indicated that p-aminobenzoic acid was more active at low pH than at high pH. Uptake studies showed that the uptake of p-aminobenzoic acid by E. coli was markedly decreased as the pH values increased. Electron micrographs of E. coli cells grown in the presence of p-aminobenzoic acid indicate that p-aminobenzoic acid caused marked damage to the cell envelope. It is suggested that p-aminobenzoic acid has at least two mechanisms of action: one mechanism in common with other organic acids and the other mechanism by interfering with the synthesis of the peptidoglycan layer by an action on the dihydrofolate reductase enzyme.
将对氨基苯甲酸对单核细胞增生李斯特菌、肠炎沙门氏菌和大肠杆菌的抗菌活性与常用酸化剂（甲酸、丙酸、乙酸、乳酸和柠檬酸）的活性进行了比较。活菌计数评估和最低抑菌浓度测定表明，对氨基苯甲酸比其他有机酸具有更强的抑制作用。对氨基苯甲酸在选定pH值下对受试微生物生长的活性表明，对氨基苯甲酸在低pH值下比在高pH值下更具活性。摄取研究表明，随着pH值升高，大肠杆菌对氨基苯甲酸的摄取显著降低。在对氨基苯甲酸存在下生长的大肠杆菌细胞的电子显微镜照片表明，对氨基苯甲酸对细胞膜造成了显著损伤。有人认为，对氨基苯甲酸至少有两种作用机制：一种与其他有机酸相同，另一种是通过作用于二氢叶酸还原酶干扰肽聚糖层的合成。
