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沙眼衣原体RNA聚合酶α亚基：序列与结构分析

Chlamydia trachomatis RNA polymerase alpha subunit: sequence and structural analysis.

作者信息

Gu L, Wenman W M, Remacha M, Meuser R, Coffin J, Kaul R

机构信息

Department of Pediatrics, University of Alberta, Edmonton, Canada.

出版信息

J Bacteriol. 1995 May;177(9):2594-601. doi: 10.1128/jb.177.9.2594-2601.1995.

DOI:10.1128/jb.177.9.2594-2601.1995
PMID:7730299
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC176926/
Abstract

We describe the cloning and sequence analysis of the region surrounding the gene for the alpha subunit of RNA polymerase from Chlamydia trachomatis. This region contains genes for proteins in the order SecY, S13, S11, alpha, and L17, which are equivalent to Escherichia coli and Bacillus subtilis r proteins. The incorporation of chlamydial alpha subunit protein into the E. coli RNA polymerase holoenzyme rather than its truncated variant lacking the amino terminus suggests the existence of structural conservation among alpha subunits from distantly related genera.

摘要

我们描述了沙眼衣原体RNA聚合酶α亚基基因周围区域的克隆和序列分析。该区域包含按SecY、S13、S11、α和L17顺序排列的蛋白质基因，它们与大肠杆菌和枯草芽孢杆菌的r蛋白等效。沙眼衣原体α亚基蛋白掺入大肠杆菌RNA聚合酶全酶中，而不是缺乏氨基末端的截短变体，这表明远缘属的α亚基之间存在结构保守性。

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