沙眼衣原体rRNA P1启动子体外转录所需序列的鉴定。
Identification of sequences necessary for transcription in vitro from the Chlamydia trachomatis rRNA P1 promoter.
作者信息
Tan M, Engel J N
机构信息
Department of Medicine, University of California, San Francisco, 94143-0654, USA.
出版信息
J Bacteriol. 1996 Dec;178(23):6975-82. doi: 10.1128/jb.178.23.6975-6982.1996.
Abstract
Chlamydia trachomatis RNA polymerase was partially purified by heparin-agarose chromatography and used in conjunction with a plasmid-borne G-less cassette template to characterize the C. trachomatis rRNA P1 promoter in vitro. Stepwise mutational analysis revealed that sequences in the -10, -25, and -35 regions are necessary for promoter activity, but no sequence upstream of position -40 is required. Partially purified C. trachomatis RNA polymerase and purified Escherichia coli holoenzyme exhibited some differences in promoter specificity.
摘要
沙眼衣原体RNA聚合酶通过肝素-琼脂糖层析进行了部分纯化，并与质粒携带的无G盒模板一起用于体外表征沙眼衣原体rRNA P1启动子。逐步突变分析表明，-10、-25和-35区域的序列对于启动子活性是必需的，但-40位置上游的序列不是必需的。部分纯化的沙眼衣原体RNA聚合酶和纯化的大肠杆菌全酶在启动子特异性上表现出一些差异。
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