Katoh M, Hirai M, Sugimura T, Terada M
Genetics Division, National Cancer Center Research Institute, Tokyo, Japan.
Oncogene. 1995 Apr 6;10(7):1447-51.
Protein kinases play a key role in cell growth regulation. We have isolated a cDNA fragment of the MST gene from the MKN28 gastric cancer cell line cDNA pool by degenerate polymerase chain reaction. MST-cDNAs were cloned from the human brain cDNA library. Nucleotide sequence analysis indicated that the MST gene encodes a novel putative non-receptor type of serine/threonine kinase with Src homology 3 (SH3) domain, two leucine zipper domains and proline rich domain. The deduced amino acid sequence corresponding to a part of kinase domain and leucine zipper domains of MST (amino acid codons 244-461) is almost identical to the published partial amino acid sequence of MLK2. MST is the first non-receptor type of serine/threonine kinase containing SH3 domain, leucine zipper domain and proline rich domain other than PTK1/Sprk. The MST gene was moderately expressed in brain, skeletal muscle and testis as a 3.8 kb mRNA, and the MST gene has been mapped to human chromosome 19q13.1-q13.2.
蛋白激酶在细胞生长调节中起关键作用。我们通过简并聚合酶链反应从MKN28胃癌细胞系cDNA文库中分离出MST基因的一个cDNA片段。从人脑cDNA文库中克隆出MST-cDNAs。核苷酸序列分析表明，MST基因编码一种新型的假定非受体型丝氨酸/苏氨酸激酶，具有Src同源3（SH3）结构域、两个亮氨酸拉链结构域和富含脯氨酸的结构域。与MST激酶结构域和亮氨酸拉链结构域的一部分相对应的推导氨基酸序列（氨基酸密码子244-461）与已发表的MLK2部分氨基酸序列几乎相同。MST是除PTK1/Sprk外第一个含有SH3结构域、亮氨酸拉链结构域和富含脯氨酸结构域的非受体型丝氨酸/苏氨酸激酶。MST基因在脑、骨骼肌和睾丸中以3.8 kb的mRNA适度表达，并且MST基因已被定位到人类染色体19q13.1-q13.2。
