• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

An improved PCR method for walking in uncloned genomic DNA.

作者信息

Siebert P D, Chenchik A, Kellogg D E, Lukyanov K A, Lukyanov S A

机构信息

Clontech Laboratories, Inc., Palo Alto, CA 94303-4607, USA.

出版信息

Nucleic Acids Res. 1995 Mar 25;23(6):1087-8. doi: 10.1093/nar/23.6.1087.

DOI:10.1093/nar/23.6.1087
PMID:7731798
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC306810/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2729/306810/290e52665b11/nar00006-0212-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2729/306810/568840780ab5/nar00006-0212-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2729/306810/290e52665b11/nar00006-0212-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2729/306810/568840780ab5/nar00006-0212-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2729/306810/290e52665b11/nar00006-0212-b.jpg

相似文献

1
An improved PCR method for walking in uncloned genomic DNA.一种用于在未克隆基因组DNA中进行步移的改进PCR方法。
Nucleic Acids Res. 1995 Mar 25;23(6):1087-8. doi: 10.1093/nar/23.6.1087.
2
Genome walking by single specific primer-polymerase chain reaction.基于单一特异性引物的聚合酶链反应进行基因组步移
Methods Enzymol. 1993;217:436-46. doi: 10.1016/0076-6879(93)17082-g.
3
One-base excess adaptor ligation method for walking uncloned genomic DNA.用于步移未克隆基因组DNA的单碱基过量接头连接法
Appl Microbiol Biotechnol. 2008 Feb;78(1):173-80. doi: 10.1007/s00253-007-1289-x. Epub 2007 Dec 11.
4
Genome walking with 2- to 4-kb steps using panhandle PCR.使用锅柄式PCR进行2至4千碱基步移的基因组步移法。
PCR Methods Appl. 1993 Feb;2(3):197-203. doi: 10.1101/gr.2.3.197.
5
Rolling circle amplification of genomic templates for inverse PCR (RCA-GIP): a method for 5'- and 3'-genome walking without anchoring.滚环扩增基因组模板用于反向 PCR(RCA-GIP):一种无需锚定进行 5' 和 3' 基因组步移的方法。
Biotechnol Lett. 2010 Jan;32(1):157-61. doi: 10.1007/s10529-009-0128-9. Epub 2009 Sep 17.
6
An A-T linker adapter polymerase chain reaction method for chromosome walking without restriction site cloning bias.一种无需限制酶克隆偏倚的 A-T 连接接头聚合酶链反应染色体步移方法。
Anal Biochem. 2012 Jun 1;425(1):62-7. doi: 10.1016/j.ab.2012.02.029. Epub 2012 Mar 8.
7
Template-blocking PCR: an advanced PCR technique for genome walking.模板阻断 PCR:一种用于基因组步移的高级 PCR 技术。
Anal Biochem. 2010 Mar 1;398(1):112-6. doi: 10.1016/j.ab.2009.11.003. Epub 2009 Nov 10.
8
Comparison and critical evaluation of PCR-mediated methods to walk along the sequence of genomic DNA.PCR介导的沿基因组DNA序列进行步移方法的比较与批判性评估。
Appl Microbiol Biotechnol. 2009 Nov;85(1):37-43. doi: 10.1007/s00253-009-2211-5.
9
Genome walking of large fragments: an improved method.大片段基因组步移:一种改进方法。
J Biotechnol. 2004 Jul 1;111(1):9-15. doi: 10.1016/j.jbiotec.2004.03.008.
10
A new approach for efficient directional genome walking using polymerase chain reaction.一种利用聚合酶链反应进行高效定向基因组步移的新方法。
Anal Biochem. 2002 Jul 1;306(1):154-8. doi: 10.1006/abio.2002.5645.

引用本文的文献

1
Center Degenerated Walking-Primer PCR: A Novel and Universal Genome-Walking Method.中心退化步移引物PCR:一种新型通用的基因组步移方法
Curr Issues Mol Biol. 2025 Aug 1;47(8):602. doi: 10.3390/cimb47080602.
2
Arbitrarily Suffixed Sequence-Specific Primer PCR for Reliable Genome Walking: Taking Genome Walkings of brevis And Rice as Examples.用于可靠基因组步移的任意加尾序列特异性引物PCR:以短柄草和水稻的基因组步移为例
Iran J Biotechnol. 2024 Oct 1;22(4):e3896. doi: 10.30498/ijb.2024.449960.3896. eCollection 2024 Oct.
3
Ultra-sensitive detection of transposon insertions across multiple families by transposable element display sequencing.

本文引用的文献

1
PCR with end trimming and cassette ligation: a rapid method to clone exon-intron boundaries and a 5'-upstream sequence of genomic DNA based on a cDNA sequence.
PCR Methods Appl. 1994 Aug;4(1):19-25. doi: 10.1101/gr.4.1.19.
2
Effective amplification of long targets from cloned inserts and human genomic DNA.从克隆插入片段和人类基因组DNA中有效扩增长片段靶标。
Proc Natl Acad Sci U S A. 1994 Jun 7;91(12):5695-9. doi: 10.1073/pnas.91.12.5695.
3
PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates.从λ噬菌体模板中以高保真度和高产量进行高达35千碱基DNA的PCR扩增。
通过转座元件展示测序对多个家族中转座子插入进行超灵敏检测。
Genome Biol. 2025 Mar 6;26(1):48. doi: 10.1186/s13059-025-03512-x.
4
Multiplex generation and single-cell analysis of structural variants in mammalian genomes.哺乳动物基因组结构变异的多重生成与单细胞分析
Science. 2025 Jan 31;387(6733):eado5978. doi: 10.1126/science.ado5978.
5
N-Ended Walker PCR: An Efficient Genome-Walking Tool.N端步移PCR:一种高效的基因组步移工具。
Biochem Genet. 2024 Jul 30. doi: 10.1007/s10528-024-10896-1.
6
Multiplex generation and single cell analysis of structural variants in a mammalian genome.哺乳动物基因组结构变异的多重生成与单细胞分析
bioRxiv. 2024 Feb 12:2024.01.22.576756. doi: 10.1101/2024.01.22.576756.
7
Fork PCR: a universal and efficient genome-walking tool.叉头PCR:一种通用且高效的基因组步移工具。
Front Microbiol. 2023 Sep 22;14:1265580. doi: 10.3389/fmicb.2023.1265580. eCollection 2023.
8
A cryptic microdeletion del(12)(p11.21p11.23) within an unbalanced translocation t(7;12)(q21.13;q23.1) implicates new candidate loci for intellectual disability and Kallmann syndrome.在不平衡易位t(7;12)(q21.13;q23.1)内的隐匿性微缺失del(12)(p11.21p11.23)暗示了智力障碍和卡尔曼综合征的新候选基因座。
Sci Rep. 2023 Aug 10;13(1):12984. doi: 10.1038/s41598-023-40037-4.
9
A microdeletion del(12)(p11.21p11.23) with a cryptic unbalanced translocation t(7;12)(q21.13;q23.1) implicates new candidate loci for intellectual disability and Kallmann syndrome.一个伴有隐匿性不平衡易位t(7;12)(q21.13;q23.1)的微缺失del(12)(p11.21p11.23)暗示了智力障碍和卡尔曼综合征的新候选基因座。
Res Sq. 2023 Mar 27:rs.3.rs-2572736. doi: 10.21203/rs.3.rs-2572736/v1.
10
Bridging PCR: An Efficient and Reliable Scheme Implemented for Genome-Walking.衔接PCR:一种用于基因组步移的高效可靠方案
Curr Issues Mol Biol. 2023 Jan 5;45(1):501-511. doi: 10.3390/cimb45010033.
Proc Natl Acad Sci U S A. 1994 Mar 15;91(6):2216-20. doi: 10.1073/pnas.91.6.2216.
4
Gene walking by unpredictably primed PCR.通过随机引物PCR进行基因步移。
Nucleic Acids Res. 1994 Aug 11;22(15):3247-8. doi: 10.1093/nar/22.15.3247.
5
Genome walking with 2- to 4-kb steps using panhandle PCR.使用锅柄式PCR进行2至4千碱基步移的基因组步移法。
PCR Methods Appl. 1993 Feb;2(3):197-203. doi: 10.1101/gr.2.3.197.
6
The human tissue plasminogen activator gene.人组织型纤溶酶原激活剂基因
J Biol Chem. 1986 May 25;261(15):6972-85.
7
Genetic applications of an inverse polymerase chain reaction.反向聚合酶链反应的遗传学应用
Genetics. 1988 Nov;120(3):621-3. doi: 10.1093/genetics/120.3.621.
8
Genomic walking and sequencing by oligo-cassette mediated polymerase chain reaction.基于寡核苷酸盒介导的聚合酶链反应的基因组步移与测序
Nucleic Acids Res. 1990 May 25;18(10):3095-6. doi: 10.1093/nar/18.10.3095.
9
A novel, rapid method for the isolation of terminal sequences from yeast artificial chromosome (YAC) clones.一种从酵母人工染色体(YAC)克隆中分离末端序列的新颖、快速方法。
Nucleic Acids Res. 1990 May 25;18(10):2887-90. doi: 10.1093/nar/18.10.2887.
10
Targeted gene walking polymerase chain reaction.靶向基因步移聚合酶链反应
Nucleic Acids Res. 1991 Jun 11;19(11):3055-60. doi: 10.1093/nar/19.11.3055.