Montenegro T, Gilman R H, Castillo R, Tsang V, Brandt J, Guevara A, Sanabria H, Verastegui M, Sterling C, Miranda E
Depto. Microbiologia, Universidad Peruana Cayetano Heredia, Lima-Peru.
Rev Inst Med Trop Sao Paulo. 1994 Jul-Aug;36(4):327-34. doi: 10.1590/s0036-46651994000400005.
Sera from patients infected with Taenia solium, Hymenolepis nana and Echinococcus granulosus were tested against homologous and heterologous parasite antigens using an ELISA assay, and a high degree of cross-reactivity was verified. To identify polypeptides responsible for this cross reactivity, the Enzyme Linked Immunoelectro Transfer Blot (EITB) was used. Sera from infected patients with T.solium, H.nana, and E.granulosus were assessed against crude, ammonium sulphate precipitated (TSASP), and lentil-lectin purified antigens of T.solium and crude antigens of H.nana and E.granulosus. Several bands, recognized by sera from patients with T.solium, H.nana, and E.granulosus infections, were common to either two or all three cestodes. Unique reactive bands in H.nana were noted at 49 and 66 K-Da and in E.granulosus at 17-21 K-Da and at 27-32 K-Da. In the crude cysticercosis extract, a specific non glycoprotein band was present at 61-67 K-Da in addiction to specific glycoprotein bands of 50, 42, 24, 21, 18, 14, and 13 K-Da. None of the sera from patients with H.nana or E.granulosus infection cross reacted with these seven glycoprotein bands considered specific for T.solium infection.
采用酶联免疫吸附测定（ELISA）对感染猪带绦虫、微小膜壳绦虫和细粒棘球绦虫患者的血清进行同源和异源寄生虫抗原检测，证实存在高度交叉反应性。为鉴定导致这种交叉反应的多肽，采用了酶联免疫电转移印迹法（EITB）。用猪带绦虫的粗抗原、硫酸铵沉淀抗原（TSASP）、扁豆凝集素纯化抗原以及微小膜壳绦虫和细粒棘球绦虫的粗抗原对感染猪带绦虫、微小膜壳绦虫和细粒棘球绦虫患者的血清进行评估。猪带绦虫、微小膜壳绦虫和细粒棘球绦虫感染患者血清识别的几条条带在两种或三种绦虫中是共有的。在微小膜壳绦虫中，49 kDa和66 kDa处以及在细粒棘球绦虫中17 - 21 kDa和27 - 32 kDa处有独特的反应条带。在囊尾蚴病粗提物中，除了50、42、24、21、18、14和13 kDa的特异性糖蛋白条带外，在61 - 67 kDa处还有一条特异性非糖蛋白条带。微小膜壳绦虫或细粒棘球绦虫感染患者的血清均未与这7条被认为是猪带绦虫感染特异性的糖蛋白条带发生交叉反应。
