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[通过免疫印迹法识别猪带绦虫及其幼虫的抗原]

[Recognition by immunoelectroblotting of antigens from Taenia solium and its larva].

作者信息

Monroy Ostria A, Gómez Gutiérrez L J, Ramírez Ramírez A, Carrillo Landín G

机构信息

Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, I.P.N. Carpio y Plan de Ayala, México, D.F., México.

出版信息

Rev Latinoam Microbiol. 1992 Jan-Mar;34(1):33-8.

PMID:1345301
Abstract

Golden hamster (Mesocricetus auratus) were infected with cysticerci of Taenia solium. Groups of three were bled and killed weekly up to 15 weeks recording the number of implanted adults in the small intestine. A longitudinal study on the antibody response against adult and larvae Ags was carried out, as well as against Ags of H. nana. By ELISA, antibodies against larvae and adult Ags of T. solium were detected since the first week, showing a peak at 3 and 5 weeks respectively. At 14 weeks antibodies levels were very low, which is the time when the parasite is eliminated. The response against Ags of H. nana was very low and disappeared at 4 weeks. By western blot we found that the infected hamsters can distinguish specific stage Ags both in the adult and larvae of T. solium. After 12 weeks only the adult was recognized, the Ags of H. nana were recognize in western blot only during the first week.

摘要

将金黄仓鼠(Mesocricetus auratus)感染猪带绦虫囊尾蚴。每组三只仓鼠,每周放血并处死,直至15周,记录小肠内植入成虫的数量。开展了一项针对成虫和幼虫抗原以及微小膜壳绦虫抗原的抗体反应的纵向研究。通过酶联免疫吸附测定法(ELISA),自第一周起就检测到了针对猪带绦虫幼虫和成虫抗原的抗体,分别在第3周和第5周出现峰值。在第14周时抗体水平非常低,此时寄生虫被清除。针对微小膜壳绦虫抗原的反应非常低,并在第4周消失。通过蛋白质印迹法,我们发现受感染的仓鼠能够区分猪带绦虫成虫和幼虫的特定阶段抗原。12周后只识别出成虫,微小膜壳绦虫的抗原仅在第一周的蛋白质印迹法中被识别。

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