Ma J K, Hiatt A, Hein M, Vine N D, Wang F, Stabila P, van Dolleweerd C, Mostov K, Lehner T
Department of Immunology, United Medical School, Guy's Hospital, London, UK.
Science. 1995 May 5;268(5211):716-9. doi: 10.1126/science.7732380.
Four transgenic Nicotiana tabacum plants were generated that expressed a murine monoclonal antibody kappa chain, a hybrid immunoglobulin A-G heavy chain, a murine joining chain, and a rabbit secretory component, respectively. Successive sexual crosses between these plants and filial recombinants resulted in plants that expressed all four protein chains simultaneously. These chains were assembled into a functional, high molecular weight secretory immunoglobulin that recognized the native streptococcal antigen I/II cell surface adhesion molecule. In plants, single cells are able to assemble secretory antibodies, whereas two different cell types are required in mammals. Transgenic plants may be suitable for large-scale production of recombinant secretory immunoglobulin A for passive mucosal immunotherapy. Plant cells also possess the requisite mechanisms for assembly and expression of other complex recombinant protein molecules.
培育出了四株转基因烟草植株，它们分别表达鼠单克隆抗体κ链、杂交免疫球蛋白A-G重链、鼠连接链和兔分泌成分。这些植株与子代重组体之间的连续有性杂交产生了同时表达所有四条蛋白链的植株。这些链组装成了一种功能性的高分子量分泌型免疫球蛋白，它能识别天然的链球菌抗原I/II细胞表面粘附分子。在植物中，单个细胞就能组装分泌型抗体，而在哺乳动物中则需要两种不同的细胞类型。转基因植物可能适合大规模生产用于被动黏膜免疫治疗的重组分泌型免疫球蛋白A。植物细胞也具备组装和表达其他复杂重组蛋白分子的必要机制。
