Dutia B M, MacCarthy-Morrogh L, Glass E J, Knowles G, Spooner R L, Hopkins J
Department of Veterinary Pathology, University of Edinburgh, Summerhall, UK.
Anim Genet. 1995 Apr;26(2):111-4. doi: 10.1111/j.1365-2052.1995.tb02643.x.
We have used a panel of anti-major histocompatibility complex (MHC) class II monoclonal antibodies (mAbs) and have assessed their specificity for the products of the individual bovine MHC (BoLA) class II subregions. The mAbs identified two distinct class II molecules by affinity purification and ELISA. Two-dimensional immunoblotting confirmed these data and NH2-terminal sequencing of the purified class II alpha chains of one member of each group identified the subregion specificity of the mAbs. The mAbs VPM36, TH22A and TH81A are specific for BoLA DQ, whereas VPM54, TH14B and J11 are specific for BoLA DR. SW73.2 reacts with both MHC subgroups of all cattle tested.
我们使用了一组抗主要组织相容性复合体（MHC）II类单克隆抗体（mAb），并评估了它们对单个牛MHC（BoLA）II类亚区产物的特异性。通过亲和纯化和酶联免疫吸附测定，这些单克隆抗体鉴定出两种不同的II类分子。二维免疫印迹证实了这些数据，并且对每组中一个成员的纯化II类α链进行氨基末端测序确定了单克隆抗体的亚区特异性。单克隆抗体VPM36、TH22A和TH81A对BoLA DQ具有特异性，而VPM54、TH14B和J11对BoLA DR具有特异性。SW73.2与所有测试牛的两个MHC亚组都发生反应。
