Massalha H, Chandra A, Hofmann D, Martin B, Lerch A, Chandra P
Laboratorium für Molekularbiologie (ZBC), Johann Wolfgang Goethe-Universität Frankfurt am Main, Germany.
Anticancer Res. 1995 Jan-Feb;15(1):117-20.
The Ymnu cell line established by us from peritoneal exudate cells of mice treated with methylnitrosourea is of macrophage origin. We have shown that 54% of these cells possess Fc-gamma receptors and can bind opsonized erythrocytes; 30% of these cells express the nonspecific esterase and 65% the Mac-1 antigen, indicating these cells are dedifferentiated. Treatment of the cells with various differentiation inducers led to time-dependent redifferentiation of the cells. The expression of the nonspecific esterase increased to 51.1% (TPA), 42.5% (RA), 63.6% (DMSO), 40.6% (SB). The fraction of Mac-1 positive cells increased to 90.5% (TPA), 80.6% (RA), 84.5% (SB) and decreased to 52.7% (DMSO). The maximal effects of the chemicals on expression of these two parameters were achieved at different times following treatment. While RA and SB were effective after one day, the maximum effect of TPA was seen at day 5.
我们从用甲基亚硝基脲处理过的小鼠腹腔渗出细胞中建立的Ymnu细胞系起源于巨噬细胞。我们已经表明，这些细胞中有54%具有Fc-γ受体，能够结合调理素化红细胞；这些细胞中有30%表达非特异性酯酶，65%表达Mac-1抗原，表明这些细胞发生了去分化。用各种分化诱导剂处理这些细胞导致细胞随时间重新分化。非特异性酯酶的表达增加到51.1%（佛波酯）、42.5%（视黄酸）、63.6%（二甲基亚砜）、40.6%（苯丁酸钠）。Mac-1阳性细胞的比例增加到90.5%（佛波酯）、80.6%（视黄酸）、84.5%（苯丁酸钠），并降低到52.7%（二甲基亚砜）。这些化学物质对这两个参数表达的最大作用在处理后的不同时间达到。视黄酸和苯丁酸钠在一天后有效，而佛波酯的最大作用在第5天出现。
