Massalha H, Chandra A, Hofmann D, Martin B, Lerch A, Chandra P
Laboratorium für Molekularbiologie (ZBC), Klinikum der Johann Wolfgang Goethe-Universität Frankfurt am Main, Germany.
Anticancer Res. 1994 Sep-Oct;14(5A):1983-90.
Here we describe the establishment of a cell line from peritoneal exudate cells for NMRI-mice treated with methylnitroso urea, which we designate Ymnu. Tests for macrophage specific characteristics have shown that 54% of Ymnu cells possess Fc-gamma receptors, 30% are nonspecific esterase positive and 65% possess the macrophage specific antigen Mac-1, indicating their macrophage origin. Although of macrophage origin, the majority of these cells are round, 13 microns in diameter. The cells grow partially in suspension and have doubling times varying from 3 to 1.75 days depending on serum concentration. Cultures of Ymnu cells achieve after 14 days densities 4 times higher than those achieved by NIH-3T3 cells. Ymnu cells have lost the anchorage dependence of growth and grew very well on semi-solid media. In addition, they possess an oncogenic potential and build tumors when injected subcutaneously in nude mice.
在此，我们描述了从用甲基亚硝基脲处理的NMRI小鼠的腹腔渗出细胞建立细胞系的过程，我们将其命名为Ymnu。巨噬细胞特异性特征检测表明，54%的Ymnu细胞具有Fc-γ受体，30%非特异性酯酶呈阳性，65%具有巨噬细胞特异性抗原Mac-1，表明它们起源于巨噬细胞。尽管起源于巨噬细胞，但这些细胞大多数呈圆形，直径为13微米。细胞部分悬浮生长，倍增时间根据血清浓度在3至1.75天之间变化。Ymnu细胞培养14天后的密度比NIH-3T3细胞高4倍。Ymnu细胞已失去对贴壁生长的依赖性，在半固体培养基上生长良好。此外，它们具有致癌潜力，皮下注射到裸鼠体内时会形成肿瘤。
