Chen M F, Chen L T, Boyce H W
Department of Internal Medicine, University of South Florida College of Medicine, Tampa 33612-4799, USA.
Anticancer Res. 1995 Jan-Feb;15(1):163-7.
Little is known whether diet or certain components in the diet can modulate the efficacy of 5-fluorouracil (5FU) in patients with colon carcinoma. Glutathione (GSH), an important antioxidant and anticarcinogen, is present in many foods in varying amounts. This study examined whether a moderately increased or decreased cellular GSH level had any effect on the growth of human colon adenocarcinoma cells HT-29 and on the cytotoxic activity of 5FU in these cells. GSH and buthionine sulfoximine were used to enhance or reduce the GSH level respectively in these cells. A 34% increase in cellular GSH level had no effect on the growth of HT-29 cells, nor on the cytotoxic activity of 5FU as determined by the MTT colorimetric assay and cell counts. A 50% reduction in the cellular GSH level was found to enhance 5FU cytotoxicity by 20% to 31% as determined by the MTT colorimetric assay, depending on the 5FU concentration. This study shows that a moderate change in the GSH level in HT-29 cells had little or no effect on the cells' growth, but a decrease in cellular GSH level slightly enhanced the cytotoxic activity of 5FU in these cells.
饮食或饮食中的某些成分是否能调节5-氟尿嘧啶（5FU）对结肠癌患者的疗效，目前所知甚少。谷胱甘肽（GSH）是一种重要的抗氧化剂和抗癌剂，在许多食物中含量各异。本研究检测了细胞内GSH水平适度升高或降低是否会对人结肠腺癌细胞HT-29的生长以及5FU对这些细胞的细胞毒活性产生影响。在这些细胞中分别使用GSH和丁硫氨酸亚砜胺来提高或降低GSH水平。细胞内GSH水平升高34%对HT-29细胞的生长以及通过MTT比色法和细胞计数测定的5FU细胞毒活性均无影响。通过MTT比色法测定，发现细胞内GSH水平降低50%可使5FU细胞毒性增强20%至31%，具体增强幅度取决于5FU的浓度。本研究表明，HT-29细胞中GSH水平的适度变化对细胞生长几乎没有影响，但细胞内GSH水平降低会略微增强5FU对这些细胞的细胞毒活性。
