神经元表达的POU家族转录因子对单纯疱疹病毒立即早期基因启动子的激活作用。
Activation of the herpes simplex virus immediate-early gene promoters by neuronally expressed POU family transcription factors.
作者信息
Lillycrop K A, Liu Y Z, Theil T, Möröy T, Latchman D S
机构信息
Department of Molecular Pathology, University College London Medical School, U.K.
出版信息
Biochem J. 1995 Apr 15;307 ( Pt 2)(Pt 2):581-4. doi: 10.1042/bj3070581.
Abstract
Herpes simplex virus immediate-early (IE) promoters contain the TAATGARAT motif which acts as a target site for the cellular POU family transcription factors Oct-1 and Oct-2. Here we show that other members of the POU family that are expressed in sensory neurons can also affect IE promoter activity. In particular, two members of the Brn-3 family of POU proteins Brn-3a and Brn-3c can activate the IE-1 and IE-3 promoters when co-transfected into fibroblasts and neuronal cells whereas a third member Brn-3b represses IE promoter activity. In contrast, Brn-3 proteins cannot overcome the inhibitory effect of neuronal Oct-2 on IE promoter activity in co-transfections nor do they prevent transactivation of the IE promoters by the Oct-1-Vmw65 complex. The potential regulation of the IE promoters by several different neuronally expressed POU proteins during the initiation, maintenance and re-activation of latent infection in sensory neurons is discussed.
摘要
单纯疱疹病毒立即早期（IE）启动子含有TAATGARAT基序，该基序作为细胞POU家族转录因子Oct-1和Oct-2的靶位点。在此我们表明，在感觉神经元中表达的POU家族的其他成员也可影响IE启动子活性。特别是，POU蛋白Brn-3家族的两个成员Brn-3a和Brn-3c在共转染到成纤维细胞和神经元细胞中时可激活IE-1和IE-3启动子，而第三个成员Brn-3b则抑制IE启动子活性。相反，在共转染中，Brn-3蛋白不能克服神经元Oct-2对IE启动子活性的抑制作用，它们也不能阻止Oct-1-Vmw65复合物对IE启动子的反式激活。本文讨论了在感觉神经元潜伏感染的起始、维持和重新激活过程中，几种不同的神经元表达的POU蛋白对IE启动子的潜在调控作用。
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