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密切相关的POU家族转录因子Brn-3a和Brn-3b对靶启动子活性的相反拮抗作用取决于POU结构域的差异。

The opposite and antagonistic effects of the closely related POU family transcription factors Brn-3a and Brn-3b on the activity of a target promoter are dependent on differences in the POU domain.

作者信息

Morris P J, Theil T, Ring C J, Lillycrop K A, Moroy T, Latchman D S

机构信息

Department of Molecular Pathology, University College London Medical School, United Kingdom.

出版信息

Mol Cell Biol. 1994 Oct;14(10):6907-14. doi: 10.1128/mcb.14.10.6907-6914.1994.

DOI:10.1128/mcb.14.10.6907-6914.1994
PMID:7935408
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC359221/
Abstract

The Brn-3a, Brn-3b, and Brn-3c POU family transcription factors are closely related to one another and are members of the group IV subfamily of POU factors. Here we show that despite this close relationship, the factors have different effects on the activity of a target promoter: Brn-3a and Brn-3c stimulate the promoter whereas Brn-3b represses it. Moreover, Brn-3b can antagonize the stimulatory effect of Brn-3a on promoter activity and can also inhibit promoter activation by the Oct-2.1 POU factor. The difference in the transactivation activities of Brn-3a and Brn-3b is dependent upon the C-terminal region containing the POU domain of the two proteins, since exchange of this domain between the two factors converts Brn-3a into a repressor and Brn-3b into an activator.

摘要

Brn-3a、Brn-3b和Brn-3c POU家族转录因子彼此密切相关，是POU因子IV亚家族的成员。在此我们表明，尽管存在这种密切关系，但这些因子对靶启动子的活性具有不同影响：Brn-3a和Brn-3c刺激启动子，而Brn-3b抑制启动子。此外，Brn-3b可拮抗Brn-3a对启动子活性的刺激作用，还可抑制Oct-2.1 POU因子介导的启动子激活。Brn-3a和Brn-3b反式激活活性的差异取决于包含这两种蛋白质POU结构域的C末端区域，因为这两个因子之间该结构域的交换可将Brn-3a转变为阻遏物，将Brn-3b转变为激活物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e65f/359221/760cbf579efe/molcellb00010-0516-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e65f/359221/abb9f316d1bf/molcellb00010-0512-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e65f/359221/8606d66105be/molcellb00010-0512-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e65f/359221/760cbf579efe/molcellb00010-0516-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e65f/359221/abb9f316d1bf/molcellb00010-0512-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e65f/359221/8606d66105be/molcellb00010-0512-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e65f/359221/760cbf579efe/molcellb00010-0516-a.jpg

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