Matthews N, Chalmers P J, Flannery G R, Nairn R C
Br J Cancer. 1976 Mar;33(3):279-89. doi: 10.1038/bjc.1976.41.
Splenocytes from inbred Wistar rats bearing a syngeneic squamous cell carcinoma (Spl) were fractionated by several techniques to characterize the lymphoid cells cytotoxic to the tumour in vitro. The anti-tumour cytotoxicity is presumably mediated primarily by T lymphocytes because it was greatly reduced by removal of T lymphocytes with heterologous anti-T serum plus complement but not by removal of other cell types. Cytotoxicity could be blocked at the tumour cell but not at the effector cell by sera taken late in tumour growth. Sera taken earlier in tumour growth could induce cytolysis of tumour cells by normal splenocytes but only if the tumour cells were treated with serum and washed before addition of the effector cells. Although splenocytes from normal and tumour-bearing rats were equally effective at lysing antibody-coated target cells it is unlikely that this mechanism is important in vivo as sera from early in tumour growth onwards contained factors (immune complexes?) which inhibited antibody-induced lymphocytolysis.
对患有同基因鳞状细胞癌的近交系Wistar大鼠的脾细胞（Spl）采用多种技术进行分离，以鉴定体外对肿瘤具有细胞毒性的淋巴细胞。抗肿瘤细胞毒性大概主要由T淋巴细胞介导，因为用异源抗T血清加补体去除T淋巴细胞后，细胞毒性大大降低，而去除其他细胞类型则不会。肿瘤生长后期采集的血清可在肿瘤细胞处阻断细胞毒性，但不能在效应细胞处阻断。肿瘤生长早期采集的血清可诱导正常脾细胞对肿瘤细胞进行细胞溶解，但前提是在加入效应细胞之前先用血清处理肿瘤细胞并洗涤。尽管来自正常大鼠和荷瘤大鼠的脾细胞在裂解抗体包被的靶细胞方面同样有效，但这种机制在体内不太可能起重要作用，因为从肿瘤生长早期开始的血清中含有抑制抗体诱导的淋巴细胞溶解的因子（免疫复合物？）。
