Nairn R C, Jablonka I M, Rolland J M, Halliday G M, Ward H A
Immunology. 1979 Feb;36(2):235-40.
Fresh rat and mouse lymphoid cells have been labelled by stable linkage with tetramethylrhodamine isothiocyanate (TMRITC). A change in intensity, either an increase or decrease of the fluorescent emission of the cells, detected by microfluorimetry, was induced by mitogen stimulation or the mixed lymphocyte reaction. The change in fluorescence was observed within 3 h of mitogen stimulation and within 0.5 h in the mixed lymphocyte test. These early cellular responses were detectable consistently whether the labelling was done before or after mitogen stimulation; post-labelling only was studied in the mixed lymphocyte reaction. The method should provide a time-saving practical procedure for early detection of the lymphoid cell responses and would readily lend itself to flow cytofluorimetry for possible routine diagnostic use.
新鲜的大鼠和小鼠淋巴细胞已通过与异硫氰酸四甲基罗丹明（TMRITC）稳定连接进行了标记。通过微量荧光测定法检测到，有丝分裂原刺激或混合淋巴细胞反应会诱导细胞荧光强度发生变化，即细胞荧光发射增加或减少。在有丝分裂原刺激后3小时内以及混合淋巴细胞试验的0.5小时内观察到了荧光变化。无论标记是在有丝分裂原刺激之前还是之后进行，这些早期细胞反应都能持续检测到；在混合淋巴细胞反应中仅研究了标记后情况。该方法应为早期检测淋巴细胞反应提供一种节省时间的实用程序，并且很容易适用于流式细胞荧光测定法，以便可能用于常规诊断。
