Riggs J E, Sirken G R, Prior L G, Hobbs M V
Department of Biology, Rider College, Lawrenceville, NJ 08648-3099, USA.
J Autoimmun. 1995 Feb;8(1):21-31. doi: 10.1006/jaut.1995.0002.
Previous studies have shown that neonatal, but not adult, murine thymic T cells proliferate when co-cultured with syngeneic, adult splenic B cells. Evidence suggests that expansion of such self-reactive T cells precedes their clonal deletion or functional inactivation (anergy). This mechanism may be of particular significance for establishing peripheral (extrathymic) tolerance. In order to assess the autostimulatory capacity of B cells present in a variety of lymphoid tissues, neonatal T cells were cultured with Peyer's patch and peritoneal cavity cells. The results indicate that B cells in these tissues readily induce T cell proliferation. Evidence suggests that the B-1 B cell subpopulation is not obligatory for this process. Self-reactive T cells were evident in the spleen of young mice, substantiating a role for B cells in maintaining peripheral tolerance. The results suggest that B cells involved in this process are distributed in a variety of lymphoid organs.
先前的研究表明，新生小鼠而非成年小鼠的胸腺T细胞在与同基因成年脾B细胞共培养时会增殖。有证据表明，此类自身反应性T细胞的扩增先于其克隆性缺失或功能失活（无反应性）。该机制对于建立外周（胸腺外）耐受性可能具有特别重要的意义。为了评估存在于多种淋巴组织中的B细胞的自身刺激能力，将新生T细胞与派尔集合淋巴结和腹腔细胞进行培养。结果表明，这些组织中的B细胞很容易诱导T细胞增殖。有证据表明，B-1 B细胞亚群并非此过程所必需。在幼鼠脾脏中可明显看到自身反应性T细胞，证实了B细胞在维持外周耐受性中的作用。结果表明，参与此过程的B细胞分布于多种淋巴器官中。
