Riggs J E, Maurio F P, Prior L G, Sirken G R, Hobbs M V
Department of Biology, Rider College, Lawrenceville, New Jersey 08648-3099.
Cell Immunol. 1994 Sep;157(2):542-8. doi: 10.1006/cimm.1994.1248.
The autologous mixed lymphocyte response (AMLR) is characterized by the proliferation of neonatal, but not adult, thymic T cells when cocultured with adult syngeneic B cells. Studies of the AMLR have revealed a temporal correlation between loss of T cell self-reactivity and development of stimulatory B cells. Such observations impart a role for B cells in the development of T cell tolerance. To test this hypothesis, we studied the AMLR in X-chromosome-linked immune-defective (XID) mice. As B cell maturation is delayed in this strain, we postulated that the AMLR-stimulatory capacity of B cells from XID mice might be impaired and lead to delayed acquisition of T cell tolerance. This report provides evidence that T cell tolerance to self-B cells is delayed in XID mice. Normal mice possess tolerant thymic T cells by 1 week of age, whereas thymic T cells in the XID mouse remain self-reactive through 4 weeks of age. These results reinforce a role for B cells in the development of T cell tolerance to self.
自体混合淋巴细胞反应（AMLR）的特征是，新生胸腺T细胞（而非成年胸腺T细胞）与成年同基因B细胞共培养时会发生增殖。对AMLR的研究揭示了T细胞自身反应性丧失与刺激性B细胞发育之间的时间相关性。这些观察结果表明B细胞在T细胞耐受性的发展中起作用。为了验证这一假设，我们研究了X染色体连锁免疫缺陷（XID）小鼠的AMLR。由于该品系中B细胞成熟延迟，我们推测XID小鼠B细胞的AMLR刺激能力可能受损，并导致T细胞耐受性的获得延迟。本报告提供了证据表明，XID小鼠对自身B细胞的T细胞耐受性延迟。正常小鼠在1周龄时就拥有耐受性胸腺T细胞，而XID小鼠的胸腺T细胞在4周龄时仍保持自身反应性。这些结果进一步证明了B细胞在T细胞对自身耐受性发展中的作用。
