Klinman D M, Haynes B F, Conover J
Section of Retroviral Immunology, Food and Drug Administration, Bethesda, Maryland 20892, USA.
AIDS Res Hum Retroviruses. 1995 Jan;11(1):97-105. doi: 10.1089/aid.1995.11.97.
Peptides were synthesized in which the type-specific determinant of the V3 loop region of gp120 (SP10) was expressed C terminal to a conserved T helper epitope (T1) on the same molecule. These T1-SP10 peptides can stimulate both cell-mediated and humoral immune responses. The current work used a novel approach to study the nature and specificity of the response elicited by these peptides. Cytokine-specific ELIspot assays were used to examine the number, kinetics and fine specificity of cells induced to secrete IL-4 and IL-6 in mice immunized with T1-SP10 peptides. Results indicate that the peptides activated cytokine-secreting cells in a dose-dependent manner in vivo. In vitro restimulation experiments demonstrated that both the SP10 and T1 regions contributed to this activation. Consistent with previous studies, mice sequentially immunized with peptides expressing different V3 loop regions generated B cell responses that were larger and more cross-reactive than those induced by a single peptide. Sequential immunizations had less effect on the number or specificity of the cytokine-producing cells.
合成了一些肽，其中gp120的V3环区域的型特异性决定簇（SP10）在同一分子上保守的T辅助细胞表位（T1）的C末端表达。这些T1-SP10肽可刺激细胞介导的免疫反应和体液免疫反应。当前的研究采用了一种新方法来研究这些肽引发的反应的性质和特异性。细胞因子特异性ELIspot分析用于检测在用T1-SP10肽免疫的小鼠中诱导分泌IL-4和IL-6的细胞的数量、动力学和精细特异性。结果表明，这些肽在体内以剂量依赖的方式激活细胞因子分泌细胞。体外再刺激实验表明，SP10和T1区域均促成了这种激活。与先前的研究一致，用表达不同V3环区域的肽顺序免疫的小鼠产生的B细胞反应比单一肽诱导的反应更大且交叉反应性更强。顺序免疫对细胞因子产生细胞的数量或特异性影响较小。
