Calabrese K S, Lagrange P H, da Costa S C
Departamento de Protozoologia, Instituto Oswaldo Cruz, Rio de Janeiro, Brasil.
Int J Exp Pathol. 1994 Dec;75(6):453-62.
Naturally immunocompromised athymic mice, neonatal mice and adult outbred OFI mice treated with the immunosuppressive agents cyclophosphamide (CY), dexamethasone (DM) and indomethacin (IM) were infected with trypomastigotes of Trypanosoma cruzi Y and CL strains. 10(4) parasites were used, except in the case of IM treatment, where mice received 10(3) trypomastigotes in one group and 10(5) in another. The course of parasitaemia, tissue distribution of amastigotes and time of mortality were compared with an infected thymus intact control group. Neonate and indomethacin treated mice presented the same pattern of parasitaemia. Death occurred as early as 9-10 days after infection. A single dose of CY 200 mg/kg given 5 days after infection enhanced the parasitaemia and increased the number of parasites in the tissues. All groups were similar in terms of colonization of the endocrine system by parasites and the adrenals showed the highest density of amastigotes nests. The thyroid gland (analysed only in neonates) showed intense amastigote accumulation. Colonization of the ovary was observed with amastigotes in both the theca interna and in the stroma. The testes (also examined only in the neonate) showed that the interstitial cells, the tunica albuginea of the seminiferous tubules and the loose connective tissue were infected. Athymic nude mice showed the most intense parasite colonization of the islets of Langerhans.
用免疫抑制剂环磷酰胺（CY）、地塞米松（DM）和吲哚美辛（IM）处理的天然免疫受损的无胸腺小鼠、新生小鼠和成年远交OFI小鼠感染了克氏锥虫Y和CL株的锥鞭毛体。除IM处理组外，每组使用10⁴个寄生虫，其中一组小鼠接受10³个锥鞭毛体，另一组接受10⁵个锥鞭毛体。将寄生虫血症的病程、无鞭毛体的组织分布和死亡时间与感染的胸腺完整对照组进行比较。新生小鼠和吲哚美辛处理的小鼠呈现相同的寄生虫血症模式。感染后9 - 10天就出现死亡。感染后5天给予单剂量200 mg/kg的CY可增强寄生虫血症，并增加组织中的寄生虫数量。所有组在寄生虫对内分泌系统的定植方面相似，肾上腺显示无鞭毛体巢的密度最高。甲状腺（仅在新生小鼠中分析）显示有强烈的无鞭毛体聚集。在卵巢的内膜和基质中均观察到无鞭毛体的定植。睾丸（也仅在新生小鼠中检查）显示间质细胞、生精小管的白膜和疏松结缔组织被感染。无胸腺裸鼠的胰岛显示出最强烈的寄生虫定植。
