Lo Y M, Noakes L, Roux E, Jeannet M, Chapuis B, Fleming K A, Wainscoat J S
Nuffield Department of Clinical Medicine, John Radcliffe Hospital, Oxford, U.K.
Br J Haematol. 1995 Mar;89(3):645-9. doi: 10.1111/j.1365-2141.1995.tb08380.x.
A highly sensitive Y-microsatellite amplification system was developed for the detection of post bone marrow transplantation (BMT) chimaerism. The system is able to detect the DNA equivalent of a single male cell in a background of 10(5) female cells. For clinical applications it has the distinct advantage that the polymorphic nature of the PCR product allows the checking of the genuineness of a positive PCR signal by reference to its allelic type. This extra degree of precision enhances the accuracy of Y-PCR for clinical diagnosis.
开发了一种高灵敏度的Y微卫星扩增系统，用于检测骨髓移植（BMT）后的嵌合体。该系统能够在10⁵个雌性细胞的背景中检测到相当于单个雄性细胞的DNA。对于临床应用，它具有明显的优势，即PCR产物的多态性允许通过参考其等位基因类型来检查阳性PCR信号的真实性。这种额外的精确度提高了Y-PCR用于临床诊断的准确性。
