Lo Y M, Roux E, Jeannet M, Chapuis B, Fleming K A, Wainscoat J S
Nuffield Department of Pathology and Bacteriology, John Radcliffe Hospital, Oxford, U.K.
Br J Haematol. 1993 Sep;85(1):223-6. doi: 10.1111/j.1365-2141.1993.tb08679.x.
We have developed a highly sensitive double amplification refractory mutation system (double ARMS) for the detection of extremely low levels of mixed chimaerism after bone marrow transplantation. The system we chose for double ARMS analysis is a highly polymorphic region 5' to the human delta-globin gene. Double ARMS analysis was found to correlate well with an established minisatellite system and was shown to demonstrate a very low level of mixed chimaerism in cases undetectable by the latter method.
我们开发了一种高度灵敏的双重扩增不应性突变系统（双重ARMS），用于检测骨髓移植后极低水平的混合嵌合体。我们选择用于双重ARMS分析的系统是人类δ-珠蛋白基因5'端的一个高度多态性区域。发现双重ARMS分析与已建立的小卫星系统相关性良好，并且在后者方法无法检测到的病例中显示出极低水平的混合嵌合体。
