Lane M E, Kalderon D
Department of Biological Sciences, Columbia University, New York, NY 1002, USA.
Mech Dev. 1995 Feb;49(3):191-200. doi: 10.1016/0925-4773(94)00317-g.
We have characterized the requirements for the Protein Kinase A (PKA) catalytic subunit, DC0, in Drosophila oogenesis. Intercellular bridges in egg chambers from PKA deficient females are unstable, leading to the formation of multinucleate nurse cells by fusions of adjacent cells. Germline clones of cells homozygous for null mutations of DC0 indicate that PKA acts autonomously in the germline. Highest levels of PKA catalytic subunit protein are associated with germ cell membranes, suggesting that targets of PKA are associated with the membrane or membrane skeleton and contribute to the stabilization of intercellular bridges. The migration of a subset of follicle cells, the border cells, is also disrupted by germline PKA mutations, implying that nurse cell junctions provide an essential path for border cell migrations.
