Trerè D, Migaldi M, Trentini G P
Dipartimento di Patologia Sperimentale, Università di Bologna, Italy.
Anal Cell Pathol. 1995 Jan;8(1):57-65.
In a series of 40 breast carcinomas, the reproducibility of two different methods for interphase AgNOR quantification was evaluated. Two operators independently defined on each slide the interphase AgNOR quantity both by measuring the area of the silver-stained structures using image cytometry and counting the AgNOR number directly at the microscope. The correlation between the values obtained by the two observers was statistically significant, but the correlation coefficient between AgNOR areas (r = 0.79; P < 0.001) was greater than that between AgNOR numbers (r = 0.38; P = 0.014). On the other hand, when interphase AgNOR area and number values obtained by each observer were compared, no significant correlation was found. This study has demonstrated that the two different methods for interphase AgNOR quantification are not comparable, and that morphometric analysis is more objective and reproducible than the counting method.
在一系列40例乳腺癌中，对两种不同的间期核仁组成区嗜银蛋白（AgNOR）定量方法的可重复性进行了评估。两名操作人员分别在每张载玻片上通过图像细胞术测量银染结构的面积以及直接在显微镜下计数AgNOR数量这两种方式来确定间期AgNOR的数量。两位观察者所获数值之间的相关性具有统计学意义，但AgNOR面积之间的相关系数（r = 0.79；P < 0.001）大于AgNOR数量之间的相关系数（r = 0.38；P = 0.014）。另一方面，当比较每位观察者获得的间期AgNOR面积和数量值时，未发现显著相关性。本研究表明，两种不同的间期AgNOR定量方法不可比，形态计量分析比计数法更客观且可重复。
