Mason-Garcia M, Harlan R E, Mallia C, Jeter J R, Steinberg H B, Fermin C, Beckman B S
Department of Anatomy, Tulane University School of Medicine, New Orleans, Louisiana, USA.
Cell Prolif. 1995 Mar;28(3):145-55. doi: 10.1111/j.1365-2184.1995.tb00063.x.
Protein kinase C (PKC) has been implicated in the signal transduction pathways for the biological effect of both interleukin-3 (IL-3) and erythropoietin (EPO) in hematopoietic target cells. The goal of this study was to identify specific classical isoforms of PKC and their localization in hematopoietic cells in response to the growth factors, IL-3 or EPO. In addition to murine fetal liver cells as a source of normal erythroid progenitor cells, we have utilized the B6SUt.EP cell line, a non-transformed hematopoietic cell line that requires IL-3 for proliferation, but for which EPO can substitute as a growth factor. With polyclonal antibodies prepared against peptide sequences specific for the alpha, beta I, beta II and gamma isoforms of PKC, we have identified beta I and beta II as the predominant nuclear isoforms in target cells that proliferate in response to IL-3 or EPO.
蛋白激酶C（PKC）参与了白细胞介素-3（IL-3）和促红细胞生成素（EPO）在造血靶细胞中产生生物学效应的信号转导途径。本研究的目的是鉴定PKC的特定经典亚型及其在造血细胞中对生长因子IL-3或EPO的响应定位。除了使用鼠胎肝细胞作为正常红系祖细胞的来源外，我们还利用了B6SUt.EP细胞系，这是一种未转化的造血细胞系，其增殖需要IL-3，但EPO可作为生长因子替代IL-3。利用针对PKC的α、βI、βII和γ亚型特异性肽序列制备的多克隆抗体，我们鉴定出βI和βII是在响应IL-3或EPO而增殖的靶细胞中的主要核亚型。
