Young H E, Mancini M L, Wright R P, Smith J C, Black A C, Reagan C R, Lucas P A
Division of Basic Medical Science, Mercer University School of Medicine, Macon, Georgia 31207, USA.
Dev Dyn. 1995 Feb;202(2):137-44. doi: 10.1002/aja.1002020205.
Previous studies have noted the presence of mesenchymal stem cells located within the connective tissue matrices of avian skeletal muscle, dermis, and heart. In these studies, clonal analysis coupled with dexamethasone treatment revealed the presence of multiple populations of stem cells composed of both lineage-committed progenitor mesenchymal stem cells and lineage-uncommitted pluripotent mesenchymal stem cells. The present study was undertaken to assess the distribution of these stem cells in the connective tissues throughout various regions of the body. Day 11 chick embryos were divided into 26 separate regions. Heart, limb skeletal muscle, and limb dermis were included as control tissues. Cells were harvested enzymatically and grown using conditions optimal for the isolation, cryopreservation, and propagation of avian mesenchymal stem cells. Cell aliquots were plated, incubated with various concentrations of dexamethasone, and examined for differentiated phenotypes. Four recurring phenotypes appeared in dexamethasone-treated stem cells: skeletal muscle myotubes, fat cells, cartilage nodules, and bone nodules. These results suggest that progenitor mesenchymal stem cells and putative pluripotent mesenchymal stem cells with the potential to form at least four tissues of mesodermal origin have a widespread distribution throughout the body, being located within the connective tissue compartments of many organs and organ systems.
先前的研究已经注意到，间充质干细胞存在于鸟类骨骼肌、真皮和心脏的结缔组织基质中。在这些研究中，克隆分析与地塞米松处理相结合，揭示了由谱系定向祖细胞间充质干细胞和谱系未定向多能间充质干细胞组成的多个干细胞群体的存在。本研究旨在评估这些干细胞在全身各个区域的结缔组织中的分布情况。将第11天的鸡胚分为26个单独的区域。心脏、肢体骨骼肌和肢体真皮作为对照组织。通过酶法收获细胞，并使用最适合鸟类间充质干细胞分离、冷冻保存和增殖的条件进行培养。将细胞等分试样接种，用不同浓度的地塞米松孵育，并检查分化表型。在地塞米松处理的干细胞中出现了四种反复出现的表型：骨骼肌肌管、脂肪细胞、软骨结节和骨结节。这些结果表明，具有形成至少四种中胚层来源组织潜力的祖细胞间充质干细胞和假定的多能间充质干细胞在全身广泛分布，存在于许多器官和器官系统的结缔组织隔室中。
