Trott D J, Masters A M, Carson J M, Ellis T M, Hampson D J
School of Veterinary Studies, Murdoch University, Western Australia.
Zentralbl Bakteriol. 1995 Jan;282(1):24-34. doi: 10.1016/s0934-8840(11)80793-0.
Multilocus enzyme electrophoresis was used to examine a collection of 41 mainly Australian isolates of Dermatophilus congolensis that had been cultured from sheep, cattle, horses, a goat, a marsupial and Chelonids. Allelic variation was examined at 16 enzyme loci. The isolates were divided into eight distinct electrophoretic types (ETs) with a mean genetic diversity per locus of 0.41. The three isolates from Chelonids represented a distinct clone in ET 1 which was separated from the remaining cluster of isolates of D. congolensis by a genetic distance of 0.852. These findings supported a previous proposal that the isolates from Chelonids represent a new species of Dermatophilus. The other 38 D. congolensis isolates were separated into two divisions (I and II) by a genetic distance of 0.560. The divisions were both subdivided into groups that either only contained alpha-hemolytic or beta-hemolytic isolates, but all isolates in each ET had only one hemolytic pattern. Isolates originating from the same animal species, or from the same geographic location, were not all closely related genetically. The allocation of isolates into ETs correlated well with their distribution into DNA restriction endonuclease analysis patterns previously established for the collection. Although relatively few distinct strains of D. congolensis were identified amongst the collection, significant genetic diversity existed within this population.
多位点酶电泳被用于检测一组41株主要来自澳大利亚的刚果嗜皮菌分离株，这些分离株取自绵羊、牛、马、一只山羊、一只有袋动物和海龟。在16个酶位点上检测等位基因变异。这些分离株被分为8种不同的电泳类型（ETs），每个位点的平均遗传多样性为0.41。来自海龟的3株分离株代表ET 1中的一个独特克隆，它与刚果嗜皮菌其余分离株簇的遗传距离为0.852。这些发现支持了之前的提议，即来自海龟的分离株代表嗜皮菌属的一个新物种。其他38株刚果嗜皮菌分离株以0.560的遗传距离分为两个组（I和II）。这两个组又进一步细分为仅包含α溶血或β溶血分离株的组，但每个ET中的所有分离株都只有一种溶血模式。来自相同动物物种或相同地理位置的分离株在遗传上并非都密切相关。将分离株分配到ETs与它们在先前为该集合建立的DNA限制性内切酶分析模式中的分布密切相关。尽管在该集合中鉴定出的刚果嗜皮菌不同菌株相对较少，但该群体中存在显著的遗传多样性。
