von Recklinghausen G, Ansorg R
Institut für Medizinische Mikrobiologie, Universität-GHS Essen, Germany.
Zentralbl Bakteriol. 1995 Jan;282(1):83-5. doi: 10.1016/s0934-8840(11)80799-1.
The bacteriostatic activity of metronidazole against Helicobacter pylori was determined with the PDM epsilometer test (E test) and with an agar dilution test. Both methods correlated in the minimum inhibitory concentration (MIC) ranges (< or = 0.5- > 32 mg/l) and in the MIC 50 (E test: 1 mg/l; agar dilution test: 2 mg/l) and MIC 90 (> 32 mg/l) values. However, comparison of test results of single strains revealed that 14 out of 105 strains (13.3%) were classified as resistant by one method but classified susceptible by the other. The correlation coefficient of 0.71 also indicated a low congruence of test results. It is concluded that both methods should be used in order to ascertain all strains resistant to metronidazole.
采用PDM比浊法（E试验）和琼脂稀释法测定了甲硝唑对幽门螺杆菌的抑菌活性。两种方法在最低抑菌浓度（MIC）范围（≤0.5～＞32mg/L）、MIC50（E试验：1mg/L；琼脂稀释法：2mg/L）和MIC90（＞32mg/L）值方面具有相关性。然而，对单株菌株测试结果的比较显示，105株菌株中有14株（13.3%）在一种方法中被判定为耐药，但在另一种方法中被判定为敏感。0.71的相关系数也表明测试结果的一致性较低。结论是，为了确定所有对甲硝唑耐药的菌株，两种方法都应使用。
