Ko H L, Winkler C, Beuth J, Tunggal L, Buss G, Jeljaszewicz J, Klippel K F, Pulverer G
Institut für Medizinische Mikrobiologie und Hygiene, Universität zu Köln, FRG.
Zentralbl Bakteriol. 1995 Jan;282(1):86-91. doi: 10.1016/s0934-8840(11)80800-5.
Inactivated cells of Propionibacterium avidum KP-40 could be shown to induce thymocyte proliferation and maturation in BALB/c-mice after intraperitoneal administration of the optimal immunomodulating dosage (1 mg per mouse). The increase in thymus weight and thymocyte numbers per mg organ weight was most pronounced and statistically significant 10 days after P. avidum KP-40 administration. Determinations of lymphatic subsets revealed a considerable up-regulation of mature cells expressing helper/inducer (L3T4+) or cytotoxic/suppressor (Lyt-2+) phenotypes and immature cells presenting both L3T4+/Lyt-2+ antigens. Obviously, P. avidum KP-40 administration accelerated murine thymocyte proliferation and maturation. Counts of BALB/c-mouse peripheral blood lymphocytes (PBL) and monocytes (PBM) revealed statistically significant increases after P. avidum KP-40 administration with peak values after 6-10 days. The determination of activated PBL (expressing interleukin-2 receptors) or PBM (expressing MAC-3 antigens) proved that P. avidum KP-40 induced a potent immunostimulation since counts of these cells were significantly enhanced after P. avidum KP-40 treatment.
在腹腔注射最佳免疫调节剂量（每只小鼠1毫克）后，可证明嗜丙酸杆菌KP-40的灭活细胞能诱导BALB/c小鼠的胸腺细胞增殖和成熟。在给予嗜丙酸杆菌KP-40后10天，胸腺重量和每毫克器官重量的胸腺细胞数量增加最为显著且具有统计学意义。淋巴细胞亚群的测定显示，表达辅助/诱导（L3T4+）或细胞毒性/抑制（Lyt-2+）表型的成熟细胞以及同时呈现L3T4+/Lyt-2+抗原的未成熟细胞有相当程度的上调。显然，给予嗜丙酸杆菌KP-40加速了小鼠胸腺细胞的增殖和成熟。对BALB/c小鼠外周血淋巴细胞（PBL）和单核细胞（PBM）的计数显示，给予嗜丙酸杆菌KP-40后有统计学意义的增加，在6 - 10天后达到峰值。对活化的PBL（表达白细胞介素-2受体）或PBM（表达MAC-3抗原）的测定证明，嗜丙酸杆菌KP-40诱导了强烈的免疫刺激，因为在嗜丙酸杆菌KP-40处理后这些细胞的计数显著增加。
