Umekawa T, Kohri K, Kurita T, Hirota S, Nomura S, Kitamura Y
Department of Urology, Kinki University School of Medicine, Osaka, Japan.
Biochem Mol Biol Int. 1995 Jan;35(1):223-30.
We investigated the expression of osteopontin (OPN) messenger (m) RNA in the rat kidney under the experimental models of several conditions that are considered to be risk factors of human renal stones. In the renal stone formation model administrated glyoxylic acid and 1,25-dihydroxyvitamin D3, pyelonephritis model and hydronephrosis model the expression of OPN mRNA in the distal convoluted tubule of the kidney was enhanced compared with the control which was sporadically positive utilizing in situ hybridization and northern blot analysis. The expression of OPN mRNA was markedly inhibited in the renal stone formation model by concominant administration of estradiol and/or progesterone.
我们在几种被认为是人类肾结石危险因素的实验模型条件下，研究了大鼠肾脏中骨桥蛋白（OPN）信使核糖核酸（mRNA）的表达。在给予乙醛酸和1,25 - 二羟基维生素D3的肾结石形成模型、肾盂肾炎模型和肾积水模型中，利用原位杂交和Northern印迹分析，与呈散在阳性的对照组相比，肾脏远曲小管中OPN mRNA的表达增强。在肾结石形成模型中，通过同时给予雌二醇和/或孕酮，OPN mRNA的表达受到显著抑制。
