Yasui T, Sato M, Fujita K, Ito Y, Nomura S, Kohri K
Department of Urology, Nagoya City University Medical School, Nagoya, Japan.
Nephron. 2001 Feb;87(2):170-6. doi: 10.1159/000045907.
The inhibitory effect of allopurinol on calcium oxalate urolithiasis has been reported, but its effect on stone matrix proteins has not been studied in vivo. To clarify the effect of allopurinol on the matrix, we investigated its effect on the expression of osteopontin (OPN), which we previously identified as an important stone matrix protein.
Control rats were not treated. Rats of the stone group were given ethylene glycol (EG) and vitamin D(3), while the allopurinol groups (low-dose group and high-dose group) were treated with allopurinol in addition to receiving EG and vitamin D(3).
The rate of renal stone formation was lower in the allopurinol groups than in the stone group. This was associated with a low expression of OPN mRNA in allopurinol-treated rats relative to that in the stone group.
Allopurinol was effective in preventing calcium oxalate stone formation and reduced OPN expression in rats. Our results suggest that allopurinol prevents renal stone formation by acting against not only the control of oxalate but also OPN expression.
已有报道称别嘌醇对草酸钙尿路结石具有抑制作用，但尚未在体内研究其对结石基质蛋白的影响。为阐明别嘌醇对基质的作用，我们研究了其对骨桥蛋白（OPN）表达的影响，我们之前已确定骨桥蛋白是一种重要的结石基质蛋白。
对照组大鼠不进行处理。结石组大鼠给予乙二醇（EG）和维生素D3，而别嘌醇组（低剂量组和高剂量组）除接受EG和维生素D3外，还给予别嘌醇治疗。
别嘌醇组的肾结石形成率低于结石组。这与别嘌醇治疗的大鼠中OPN mRNA表达低于结石组有关。
别嘌醇可有效预防草酸钙结石形成，并降低大鼠体内OPN的表达。我们的结果表明，别嘌醇不仅通过控制草酸，还通过作用于OPN的表达来预防肾结石形成。
