Hosono M, Sone N, Endo K, Saga T, Kobayashi H, Hosono M N, Sakahara H, Yasunaga K, Konishi J
Department of Nuclear Medicine, Faculty of Medicine, Kyoto University, Japan.
Nucl Med Biol. 1995 Jan;22(1):71-6. doi: 10.1016/0969-8051(94)00072-r.
To experimentally assess the kinetics of platelets in thrombocytopenia, we constructed a canine model using 111In-oxine labeled autologous platelets and an intact antiplatelet monoclonal antibody (MAb) NNKY2-11 (IgG2a). With the infusion of radiolabeled autologous platelets into dogs, the peripheral platelet count and blood radioactivity level were examined, and the radioactivity in the liver, spleen and heart was determined with scintigraphic analysis. Thereafter, i.v. injection of 100 micrograms/kg of NNKY2-11 had no effect on platelet counts or the biodistribution of radiolabeled platelets. However, 200 and 300 micrograms/kg of MAb reduced the platelets, and the radioactivity of the liver and spleen augmented clearly after injection of MAb. Platelet radioactivity in serum, which had decreased after MAb infusion, did not recover, even when peripheral platelet counts returned to the normal levels, indicating that these new platelets might be derived from the platelet-storage pool or new thrombocytogenesis. This model of antiplatelet MAb induced thrombocytopenia seems to be useful for analyzing the kinetics of platelets in thrombocytopenia.
为了通过实验评估血小板减少症中血小板的动力学，我们构建了一个犬类模型，使用铟-111 奥克辛标记的自体血小板和完整的抗血小板单克隆抗体（MAb）NNKY2-11（IgG2a）。将放射性标记的自体血小板注入犬体内后，检测外周血小板计数和血液放射性水平，并通过闪烁扫描分析测定肝脏、脾脏和心脏中的放射性。此后，静脉注射 100 微克/千克的 NNKY2-11 对血小板计数或放射性标记血小板的生物分布没有影响。然而，200 和 300 微克/千克的单克隆抗体降低了血小板数量，注射单克隆抗体后肝脏和脾脏的放射性明显增加。单克隆抗体输注后血清中的血小板放射性降低，即使外周血小板计数恢复到正常水平也未恢复，这表明这些新血小板可能来自血小板储存池或新的血小板生成。这种抗血小板单克隆抗体诱导的血小板减少症模型似乎有助于分析血小板减少症中血小板的动力学。
