Watanabe A, Kurokawa T, Sato J, Iwasa S, Ogawa Y
DDS Research Laboratories, Takeda Chemical Industries, Ltd., Osaka, Japan.
Biol Pharm Bull. 1994 Dec;17(12):1673-5. doi: 10.1248/bpb.17.1673.
An ex vivo antiviral assay was established which uses hepatocytes from mice given recombinant mouse interferon-beta (rmIFN-beta). Assay results were compared with results obtained with a 2',5'-oligoadenylate synthetase (2-5AS) assay. rmIFN-beta was intraperitoneally administered to C3H mice and the antiviral state of their liver parenchymal cells was evaluated in an in vitro cytopathic effect assay. In this assay, cells are infected with vesicular stomatitis virus (VSV) and surviving cells are determined colorimetrically. The antiviral state was measured as the resistance of hepatocytes to VSV infection with increasing doses of rmIFN-beta. The antiviral state correlated well with the dose-dependent increase in hepatic 2-5AS activity. This good correlation suggests that induction of 2-5AS mediates the antiviral action of interferon in liver tissue. This ex vivo assay could be a useful tool for estimating the ability of hepatocytes to resist hepatitis virus infection.
建立了一种体外抗病毒试验，该试验使用给予重组小鼠干扰素-β（rmIFN-β）的小鼠的肝细胞。将试验结果与通过2'，5'-寡腺苷酸合成酶（2-5AS）试验获得的结果进行比较。将rmIFN-β腹腔注射给C3H小鼠，并在体外细胞病变效应试验中评估其肝实质细胞的抗病毒状态。在该试验中，用水泡性口炎病毒（VSV）感染细胞，并通过比色法测定存活细胞。抗病毒状态通过随着rmIFN-β剂量增加肝细胞对VSV感染的抗性来衡量。抗病毒状态与肝脏2-5AS活性的剂量依赖性增加密切相关。这种良好的相关性表明2-5AS的诱导介导了干扰素在肝组织中的抗病毒作用。这种体外试验可能是评估肝细胞抵抗肝炎病毒感染能力的有用工具。
