Monteiro A N, Borojevic R
Departamento de Bioquímica, Universidade Federal do Rio de Janeiro, Brazil.
In Vitro Cell Dev Biol Anim. 1995 Feb;31(2):149-55. doi: 10.1007/BF02633975.
Liver connective tissue cells (LCTC) isolated from patients with fibrotic livers have morphological and biochemical characteristics of myofibroblasts. We have examined the proliferation of LCTC derived from normal livers and from livers with fibrosis of different etiologies, as well as proliferation of skin fibroblasts. We have compared proliferation rates in the presence of fresh human serum and heat-inactivated serum. While skin fibroblast and LCTC from normal liver showed no difference, proliferation of LCTC from fibrotic livers was markedly decreased in the presence of heat-inactivated serum. We demonstrate that the native complement component C1 is a factor involved in the induction of DNA synthesis and proliferation of LCTC isolated from fibrotic livers. We propose that native C1, acting probably in cooperation with other growth factors, is involved in the expansion of connective tissue cells during the development of liver fibrosis.
从纤维化肝脏患者中分离出的肝结缔组织细胞（LCTC）具有肌成纤维细胞的形态学和生化特征。我们研究了源自正常肝脏和不同病因纤维化肝脏的LCTC的增殖情况，以及皮肤成纤维细胞的增殖情况。我们比较了在新鲜人血清和热灭活血清存在下的增殖率。虽然皮肤成纤维细胞和正常肝脏的LCTC没有差异，但在热灭活血清存在下，纤维化肝脏的LCTC增殖明显减少。我们证明天然补体成分C1是参与诱导从纤维化肝脏分离出的LCTC的DNA合成和增殖的一个因素。我们提出，天然C1可能与其他生长因子协同作用，参与肝纤维化发展过程中结缔组织细胞的扩增。
