Dolby C A, Hannant D, Mumford J A
Department of Infectious Diseases, Animal Health Trust, Newmarket, Suffolk, UK.
Br Vet J. 1995 Jan-Feb;151(1):27-37. doi: 10.1016/s0007-1935(05)80061-2.
Five yearling ponies were vaccinated with inactivated Equid herpesvirus type 1 (EHV-1) in Freund's complete adjuvant as a double emulsion and revaccinated 6 weeks later with EHV-1 in Freund's incomplete adjuvant. These ponies and three age-matched controls were challenged intra-nasally after a further 6 weeks with homologous live virus and monitored clinically, biologically and serologically. After challenge, clinical signs were mild in both groups. No cell-associated viraemias were detected in vaccinated ponies. Vaccination induced high levels of complement-fixing (CF) and virus-neutralizing (VN) antibody, and elicited a response to all major viral glycoproteins as shown by western blot analysis.
五匹一岁小马用弗氏完全佐剂中的灭活1型马疱疹病毒（EHV-1）以双重乳剂形式进行接种，并在6周后用弗氏不完全佐剂中的EHV-1再次接种。这些小马和三匹年龄匹配的对照在再过6周后经鼻内接种同源活病毒，并进行临床、生物学和血清学监测。攻毒后，两组的临床症状均较轻。在接种疫苗的小马中未检测到细胞相关病毒血症。接种疫苗诱导产生了高水平的补体结合（CF）和病毒中和（VN）抗体，并且如蛋白质印迹分析所示，引发了对所有主要病毒糖蛋白的反应。
