Tamura K, Yamamura M, Nishigami T, Tonokatsu Y, Yamamoto I, Fukuda Y, Satomi M, Shimoyama T
Department of Internal Medicine 4, Hyogo College of Medicine, Japan.
Eur J Gastroenterol Hepatol. 1994 Dec;6 Suppl 1:S39-43.
To evaluate the effect of certain cytotoxic products of Helicobacter pylori on superoxide generation in situ and on the infiltration by polymorphonuclear cells into the gastric mucosa. The agents studied were ammonia, a culture supernatant of H. pylori, and N-formyl-methionyl-leucyl-phenylalanine (FMLP), a chemotactic factor.
One or two of these agents were dripped onto rabbit gastric epithelium. The number of polymorphonuclear cells in gastric mucosa were counted histologically. Superoxide generation was measured by a chemiluminescence method, using a Cypridina luciferin analog, 2-methyl-6-phenyl-3,7-dihydroimidazo[1,2-alpha]pyrazin-3-one , a chemiluminescence probe that responds to the generation of superoxides and singlet oxygen radicals. Bovine erythrocyte copper zinc superoxide dismutase was dripped to confirm that the increase in photon counts was a direct consequence of the generation of superoxides.
The greatest infiltration of polymorphonuclear cells into the gastric mucosa was observed with the administration of 1 mmol/l FMLP followed by 5.9 mmol/l ammonia, and 10 mmol/l FMLP followed by 59 mmol/l ammonia. There was no increase in the number of polymorphonuclear cells in the other experiments. Increased chemiluminescence was observed in conjunction with the infiltration of polymorphonuclear cells and was inhibited by superoxide dismutase.
Acute gastritis and superoxide generation in situ were induced by ammonia after pretreatment with FMLP.
评估幽门螺杆菌某些细胞毒性产物对超氧化物原位生成以及多形核细胞向胃黏膜浸润的影响。所研究的物质为氨、幽门螺杆菌培养上清液以及趋化因子N-甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP）。
将这些物质中的一种或两种滴加到兔胃上皮。组织学计数胃黏膜中的多形核细胞数量。使用海萤荧光素类似物2-甲基-6-苯基-3,7-二氢咪唑并[1,2-α]吡嗪-3-酮通过化学发光法测量超氧化物生成，该化学发光探针可对超氧化物和单线态氧自由基的生成作出反应。滴加牛红细胞铜锌超氧化物歧化酶以确认光子计数增加是超氧化物生成的直接结果。
观察到，给予1 mmol/l FMLP后再给予5.9 mmol/l氨，以及给予10 mmol/l FMLP后再给予59 mmol/l氨时，多形核细胞向胃黏膜的浸润最为明显。在其他实验中，多形核细胞数量没有增加。随着多形核细胞浸润观察到化学发光增加，且超氧化物歧化酶可抑制该现象。
FMLP预处理后，氨可诱导急性胃炎和超氧化物原位生成。
