Fukui S, Shimoyama T, Tamura K, Yamamura M, Satomi M
Fourth Department of Internal Medicine, Hyogo College of Medicine, Nishinomiya, Japan.
J Gastroenterol. 1997 Aug;32(4):464-71. doi: 10.1007/BF02934084.
As we consider succinic acid to be an exacerbating factor in ulcerative colitis, we investigated its influence on rat colonic mucosa in terms of mucosal blood flow and superoxide generation. We measured mucosal blood flow by the hydrogen gas clearance method and superoxide generation by the chemiluminescence method, and observed histopathological findings to determine the effects of succinic acid. After the instillation of succinic acid of any concentration tested to the colon, mucosal blood flow decreased. Histopathologically, the higher the concentration of succinic acid, the greater was the erosion formation in the colonic mucosa, while significant polymorpho-nuclear cell infiltration superoxide generation from colon tissue were observed with 0.01% succinic acid compared with higher or lower concentrations. Succinic acid, at fecal concentrations found in active stage ulcerative colitis, appears to be implicated in mucosal injury, mediated by a decrease in colonic mucosal blood flow and infiltration of superoxide-generating polymorpho-nuclear cells into the mucosa.
由于我们认为琥珀酸是溃疡性结肠炎的一个加重因素，因此我们从黏膜血流和超氧化物生成方面研究了其对大鼠结肠黏膜的影响。我们通过氢气清除法测量黏膜血流，通过化学发光法测量超氧化物生成，并观察组织病理学结果以确定琥珀酸的作用。在向结肠滴注任何测试浓度的琥珀酸后，黏膜血流减少。组织病理学上，琥珀酸浓度越高，结肠黏膜的糜烂形成越严重，而与较高或较低浓度相比，在0.01%琥珀酸组观察到结肠组织有明显的多形核细胞浸润和超氧化物生成。在活动期溃疡性结肠炎患者粪便中发现的琥珀酸浓度下，似乎与黏膜损伤有关，其介导机制是结肠黏膜血流减少以及产生超氧化物的多形核细胞浸润到黏膜中。
